Phospho-BLNK (Tyr96) Antibody

• 产品编号：CST-3601S      品牌：CST       原厂货号：3601S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-BLNK (Tyr96) 抗体只能检测内源的在tyr96位点磷酸化的BLNK蛋白。此多克隆抗体是通过合成人源对应的BLNK Tyr96位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。BLNK (B 细胞连接蛋白),也叫做SLP-65或BASH,是一个接头分子，在B细胞的激活和B细胞抗原受体(BCR)结合中发挥了重要的作用。BLNK在BCR相关的Syk和下游信号级联反应中发挥作用(1,2)。BLNK在N-末端有几个SH2 结合域(YXXP)并在C-末端有一个SH2结合域。BCR结合后, BLNK被Syk在多个具有YXXP域的位点磷酸化，包括Tyr72, Tyr84, Tyr96 和 Tyr178 (1)。这些磷酸化的域为信号分子（如Btk, PLCgamma和Vav）提供结合的位点。这些信号分子通过他们的SH2域结合到BLNK并一同激活下游的信号通路(3,4)。通过其SH2域, BLNK也能与酪氨酸磷酸化的目标蛋白如HPK1相互作用从而招募到BCR复合体上，参与到下游的信号通路中(5)。

1. Kurosaki, T. and Tsukada, S. (2000) Immunity 12, 1-5.
2. Fu, C. et al. (1998) Immunity 9, 93-103.
3. Ishiai, M. et al. (1999) Immunity 10, 117-125.
4. Baba, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 2582-2586.
5. Tsuji, S. et al. (2001) J. Exp. Med. 194, 529-539.

原厂资料：

Specificity / Sensitivity

Phospho-BLNK (Tyr96) Antibody detects endogenous levels of BLNK only when phosphorylated at tyrosine 96.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr96 of human BLNK. Antibodies are purified by protein A and peptide affinity chromatography.

Background

BLNK (B cell linker protein), also known as SLP-65 or BASH, is an adaptor molecule that plays key roles in B cell activation and B cell antigen receptor (BCR) engagement. BLNK acts on the interface between BCR-associated Syk and the downstream signaling cascades (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its amino-terminus and a SH2 domain at its carboxy-terminus. After BCR ligation, BLNK is phosphorylated by Syk at multiple YXXP motifs including Tyr72, Tyr84, Tyr96 and Tyr178 (1). These phosphorylated motifs provide docking sites for signaling molecules such as Btk, PLCgamma and Vav. These signaling molecules bind to BLNK through their SH2 domains and together activate downstream signaling pathways (3,4). Through its SH2 domain, BLNK can also interact with tyrosine-phosphorylated targets such as HPK1 to recruit it to the BCR complex for signaling (5).

1. Kurosaki, T. and Tsukada, S. (2000) Immunity 12, 1-5.
2. Fu, C. et al. (1998) Immunity 9, 93-103.
3. Ishiai, M. et al. (1999) Immunity 10, 117-125.
4. Baba, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 2582-2586.
5. Tsuji, S. et al. (2001) J. Exp. Med. 194, 529-539.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.