Phospho-eIF2α (Ser51) (119A11) Rabbit mAb

• 产品编号：CST-3597S      品牌：CST       原厂货号：3597S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-eIF2alpha (Ser51) RmAb检测仅在Ser51位点磷酸化的内源性eIF2alpha蛋白水平。该抗体不与其它位点磷酸化的elF2alpha蛋白发生交叉反应。通过人工合成人源eIF2alpha蛋白Ser51位点周围序列相应的磷酸化片段去免疫动物从而制备单克隆抗体。研究证明在不同的应激条件下真核生物起始因子2 (eIF2) α亚型的磷酸化可下调蛋白质的合成。eIF2蛋白与GTP以及起始tRNA（Met-tRNAi）结合，然后将Met-tRNA转移到40S核糖体亚基上共同形成43S前起始复合物(1,2)。eIF2通过eIF2B催化反应将GTP交换GDP从而促进新一轮翻译的起始(1,2)。病毒感染(PKR)、内质网应激（endoplasmic reticulum stress）(PERK/PEK)、氨基酸饥饿(GCN2)或血红素缺乏症(HRI)能够激活相关的激酶，这些激酶可以使eIF2的α亚基磷酸化(3,4)。这个磷酸化稳固eIF2-GDP-eIF2B复合物，同时期抑制eIF2B的降解。通过IFN-γ 和TNF-α的诱导的PKR能有效的诱导eIF2α蛋白在Ser51位点磷酸化(5,6)。

1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
2. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
3. Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

原厂资料：

Specificity / Sensitivity

Phospho-eIF2alpha (Ser51) RmAb detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha.

Background

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).

1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
2. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
3. Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.