LCP1 (D1C3) Rabbit mAb

• 产品编号：CST-3588S      品牌：CST       原厂货号：3588S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LCP1 (D1C3)Rabbit mAb可以识别内源性总的LCP1蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人Rb蛋白807/811位丝氨酸附近氨基酸一致。高度保守并广泛表达的plastin蛋白组成了一系列的actin结合蛋白，包括能够促进actin集合成束的蛋白。哺乳动物的三个plastins表达量不同，包括L-plastin, T-plastin, 和 I-plastin. T-plastin（plastin-3）在大部分的实体组织细胞中都有发现，I-plastin（plastin-1）特异性的在肾，结肠和小肠组织中表达（1-3）。研究发现L-plastin（plastin-2）或淋巴细胞胞浆蛋白1 (LCP1)主要在造血细胞和非造血型肿瘤中表达，在结肠癌细胞系中随着恶性程度的增加表达量也会上升（4）。研究人员发现LCP1在转移前癌细胞系中过表达会引发侵润和E-cadherin表达下降，这是转移性癌细胞系的特征（5）。T cell receptor/CD3 复合物与CD2细胞黏附分子或CD28受体一起作用磷酸化LCP1的第5位丝氨酸激活LCP1（6）。LCP1的第5位丝氨酸磷酸化会增加LCP1结合到F-actin的能力并增加细胞的运动型（7,8）。

1. Lin, C.S. et al. (1993) J Biol Chem 268, 2781-92.
2. Lin, C.S. et al. (1994) Mol Cell Biol 14, 2457-67.
3. Delanote, V. et al. (2005) Acta Pharmacol Sin 26, 769-79.
4. Otsuka, M. et al. (2001) Biochem Biophys Res Commun 289, 876-81.
5. Foran, E. et al. (2006) Int J Cancer 118, 2098-104.
6. Wabnitz, G.H. et al. (2007) Eur J Immunol 37, 649-62.
7. Janji, B. et al. (2006) J Cell Sci 119, 1947-60.
8. Klemke, M. et al. (2007) Int J Cancer 120, 2590-9.

原厂资料：

Specificity / Sensitivity

LCP1 (D1C3) Rabbit mAb recognizes endogenous levels of total LCP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp567 of human LCP1 protein.

Background

Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling. Three plastins exhibiting differential expression are found in mammals and include L-plastin, T-plastin, and I-plastin. T-plastin (plastin-3) is found in cells of most solid tissues, while I-plastin (plastin-1) is expressed specifically in the kidney, colon, and small intestine (1-3). Research studies have shown that L-plastin (plastin-2) or lymphocyte cytosolic protein 1 (LCP1) is mainly expressed in hematopoietic cells and nonhematopoietic tumors, and increased expression correlates with metastatic progression in colon cancer cell lines (4). Investigators have found that overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of E-cadherin expression, which is characteristic of metastatic cancer cell lines (5). LCP1 becomes phosphorylated at Ser5 upon stimulation through the T cell receptor/CD3 complex in association with the CD2 cell adhesion molecule or the CD28 receptor (6). Phosphorylation at Ser5 enhances the ability of LCP1 to bind to F-actin and increases cell motility (7,8).

1. Lin, C.S. et al. (1993) J Biol Chem 268, 2781-92.
2. Lin, C.S. et al. (1994) Mol Cell Biol 14, 2457-67.
3. Delanote, V. et al. (2005) Acta Pharmacol Sin 26, 769-79.
4. Otsuka, M. et al. (2001) Biochem Biophys Res Commun 289, 876-81.
5. Foran, E. et al. (2006) Int J Cancer 118, 2098-104.
6. Wabnitz, G.H. et al. (2007) Eur J Immunol 37, 649-62.
7. Janji, B. et al. (2006) J Cell Sci 119, 1947-60.
8. Klemke, M. et al. (2007) Int J Cancer 120, 2590-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.