Phospho-LAT (Tyr191) Antibody

• 产品编号：CST-3584P      品牌：Cell Signaling Technology       原厂货号：3584P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho LAT (Tyr191) Antibody只能检测内源的在tyr191位点磷酸化的LAT蛋白。此多克隆抗体是通过合成人源对应的LAT Tyr191位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。LAT是跨膜蛋白，在T 细胞， NK 细胞和柱状细胞中表达并在T细胞信号转导中作为重要的参与分子(1)。在TCR 招募下, 激活的Zap-70 磷酸化LAT 位于SH2区域的几个保守的酪氨酸残基，使这些区域暴露并作为下游信号的结合位点(2,3)。LAT在 Tyr171 和 Tyr191 位点的磷酸化使得Grb2, Gads/SLP-76, PLCgamma1 和 PI3 激酶通过SH2区域与之结合并在细胞膜上定位。这些过程最终导致了相对应的信号通路的激活(1-4)。

1. Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-6283.
2. Zhang, W. et al. (1998) Cell 92, 83-92.
3. Paz, P. E. et al. (2001) Biochem. J. 356, 461-471.
4. Zhang, W. et al. (2000) J. Biol. Chem. 275, 23355-23361.

原厂资料：

Specificity / Sensitivity

Phospho LAT (Tyr191) Antibody detects endogenous levels of LAT only when phosphorylated at tyrosine 191.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr191 of human LAT. Antibodies are purified by protein A and peptide affinity chromatography.

Background

LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr191 enables the binding of Grb2, Gads/SLP-76, PLCγ1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).

1. Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-6283.
2. Zhang, W. et al. (1998) Cell 92, 83-92.
3. Paz, P. E. et al. (2001) Biochem. J. 356, 461-471.
4. Zhang, W. et al. (2000) J. Biol. Chem. 275, 23355-23361.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.