Human CD40 Ligand (hCD40L)

• 产品编号：CST-3583SF      品牌：CST       原厂货号：3583SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > CD因子及其它细胞表面标记分子
• 应用分类：

描述：

CST在大肠杆菌细胞中表达重组的人CD40配体(hCD40L) Met113-Leu161 (Accession #P29965)蛋白。重组的hCD40L蛋白在N-末端含有标签，其分子量据推算为16,184Da。DTT-还原和未还原的蛋白是作为一个16kDa 的多肽而迁移。重组 hCD40L蛋白的N-末端MQKGD的序列通过测序得到。

有载体: 每微克hCD40L蛋白溶解在包含20 μg BSA的PBS（pH 7.2）溶液中，通过 0.22 μm 滤膜并冻干。

无载体：每微克hCD40L蛋白溶解在PBS（pH 7.2）溶液中，通过 0.22 μm 滤膜并冻干。

CD40配体(CD40L)，是TNF超家族配体中的一位成员，作为膜结合或可溶性的三聚体而表达(1)。CD40L的膜结合和可溶性的三聚体都具有生物活性(1,2)。CD40L主要是在激活的T细胞上表达，然而，肥大细胞、噬碱细胞和NK细胞也能表达CD40L(1)。CD40L的作用主要是T细胞的共刺激因子并增强T依赖的B细胞的应答(1,2)。CD40L结合到CD40，是作为受体表达在B细胞、树突状细胞和巨噬细胞上(1)。CD40L结合到CD40上导致了TRAF蛋白(TRAFs 2,3,5 和 6)的招募并使得JNK和 NF-κB通路激活(1,2)。

原厂资料：

Source / Purification

Recombinant human CD40 Ligand (hCD40L) Met113-Leu161 (Accession #P29965) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hCD40L has a Met on the amino terminus and has a calculated MW of 16,184. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptides. The expected amino terminus MQKGD of recombinant hCD40L was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCD40L. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hCD40L was determined in a cell proliferation assay using human B cells. The ED50 of each lot is between 0.5-2 μg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hCD40L.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hCD40L. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

CD40 Ligand (CD40L), a member of the TNF superfamily of ligands, is expressed as either a membrane-bound or soluble homotrimer (1). Both membrane-bound and soluble forms of CD40L have biological activity (1,2). CD40L is expressed primarily on activated T cells; however, mast cells, basophils, and NK cells may also express CD40L (1). CD40L functions as an important T cell co-stimulatory molecule and enhances T-dependent B cell responses (1,2). CD40L binds CD40, which is a receptor expressed on B cells, dendritic cells, and macrophages (1). Binding of CD40L to CD40 leads to the recruitment of TRAF proteins (TRAFs 2,3,5, and 6) resulting in the activation of the JNK and NF-κB pathways (1,2).

注意事项：

Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.