LDHA (C4B5) Rabbit mAb

• 产品编号：CST-3582S      品牌：CST       原厂货号：3582S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LDHA (C4B5) Rabbit mAb用于检测内源性LDHA总蛋白水平。单克隆抗体通过用对应于人源LDHA蛋白序列的合成肽免疫动物制备而得。乳酸脱氢酶（LDH）催化丙酮酸和NADH转化为乳酸和NAD＋的可逆反应。当线粒体产生ATP所需氧气不足时，该反应重新利用糖酵解产生的NADH，产生NAD＋，NAG＋重新进入糖酵解过程。肌肉中主要的LDH的形式是LDHA(A型同工酶)。LDHA的启动子有HIF-1-α结合位点（1）。在低氧条件下，LDHA的表达被诱导（2）。在肌肉长期巨烈运动时，当供氧不足时，乳酸就会聚集在肌肉细胞中。当氧气达到正常水平时， LDH 会将乳酸转变成丙酮酸进入三羧酸循环产生ATP。

1. Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.
2. Semenza, G.L. (2007) Biochem J 405, 1-9.

原厂资料：

Specificity / Sensitivity

LDHA (C4B5) Rabbit mAb detects endogenous levels of total LDHA protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA.

Background

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1-α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.

1. Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.
2. Semenza, G.L. (2007) Biochem J 405, 1-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.