Sox2 (D6D9) XP? Rabbit mAb

• 产品编号：CST-3579S      品牌：CST       原厂货号：3579S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Sox2 (D6D9) XP^® Rabbit mAb兔单抗识别内源性的Sox2蛋白。单抗由合成肽段免疫动物产生，相应肽段与人源的Sox2蛋白的Gly179氨基酸残基序列一致。胚胎干细胞分离自囊泡的内细胞团，并有独特的多分化潜能和自我更新潜能。Sox2是一种对于保持细胞多潜能性起关键作用的转录因子(1)。Sox2, Oct-4和Nanog在调控网络中协同作用(1-3)。表达Sox2 或 Oct-4的siRNA诱导沉默会导致细胞多潜能性的丢失(4,5)。染色质免疫沉淀实验显示Sox2和 Oct-4能结合到数千个基因的调控位点，显示了这些转录因子在胚胎早期发育中的重要性(6,7)。最近的研究发现Sox2基因在肺癌和食管鳞癌中扩增（8）.

1. Conley, B.J. et al. (2004) Int J Biochem Cell Biol 36, 555-67.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Boyer, L.A. et al. (2005) Cell 122, 947-56.
5. Loh, Y.H. et al. (2006) Nat Genet 38, 431-40.
6. Matin, M.M. et al. (2004) Stem Cells 22, 659-68.
7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
8. Okita, K. et al. (2007) Nature 448, 313-7.
9. Arnold, K. et al. (2011) Cell Stem Cell 9, 317-29.

原厂资料：

Specificity / Sensitivity

Sox2 (D6D9) XP^® Rabbit mAb detects endogenous levels of Sox2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2.

Background

Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3) and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).

1. Conley, B.J. et al. (2004) Int J Biochem Cell Biol 36, 555-67.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Boyer, L.A. et al. (2005) Cell 122, 947-56.
5. Loh, Y.H. et al. (2006) Nat Genet 38, 431-40.
6. Matin, M.M. et al. (2004) Stem Cells 22, 659-68.
7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
8. Okita, K. et al. (2007) Nature 448, 313-7.
9. Arnold, K. et al. (2011) Cell Stem Cell 9, 317-29.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.