CD44 Antibody

• 产品编号：CST-3578S      品牌：CST       原厂货号：3578S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CD44 Antibody 能够检测内源CD44蛋白总体水平。此多克隆抗体是通过合成人源对应的CD44 Leu135位点周围的肽段来免疫动物而获得。抗体是通过多肽亲和层析纯化。CD44 是I类跨膜糖蛋白，能够介导细胞-细胞和细胞-基质通过对透明质酸(HA)的亲和性或细胞外基质(ECM)而相互作用。CD44 具有多种形态，多种剪切形式，并且具有多样的转录后修饰(1,2)。细胞表面CD44表达的增加是T细胞激活的特征之一，并且此蛋白在炎症应答过程中上调表达。CD44与HER2 的相互作用与卵巢癌细胞的生长有关(1-3)。CD44 与埃兹蛋白、根蛋白和膜突蛋白(ERM)的相互作用与细胞骨架连接到细胞质膜和ECM有关(4-6)。在静息细胞中，CD44是组成型在 Ser325位点磷酸化。 PKC的激活使Ser291位点磷酸化,Ser325位点去磷酸化, 导致埃兹蛋白和CD44蛋白分离和定向运动(4)。

1. Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196.
2. Cichy, J. and Puré, E. (2003) J. Cell Biol. 161, 839-843.
3. Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913-27918.
4. Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407.
5. Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895.
6. Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.

原厂资料：

Specificity / Sensitivity

CD44 Antibody detects endogenous levels of total CD44 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Leu135 of human CD44. Antibodies are purified by peptide affinity chromatography.

Background

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).

1. Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196.
2. Cichy, J. and Puré, E. (2003) J. Cell Biol. 161, 839-843.
3. Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913-27918.
4. Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407.
5. Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895.
6. Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.