CD19 Antibody

• 产品编号：CST-3574S      品牌：CST       原厂货号：3574S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CD19 抗体能够检测内源水平CD19蛋白水平而与其磷酸化的状态无关。此多克隆抗体是通过合成人源对应的CD19 C-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。CD19是一个分子量为95 kDa的共受体, 它能在B细胞中放大级联反应的信号(1)。在B细胞的表面, CD19与CD21, CD81和 Leu-13相连并发挥其作用。CD19的细胞质尾巴有9个保守的酪氨酸在CD19介导的偶联信号分子到受体时发挥了重要的作用(1)。B 细胞受体或CD19连结后, CD19的Tyr531和Tyr500位点被逐渐的磷酸化。此磷酸化使得PI3激酶和Src家族的酪氨酸激酶偶联到CD19并激活PI3K和Src 信号通路(2,3)。B细胞受体与CD19的共结合也促进了CD19中Tyr409位点的磷酸化。在此位点的磷酸化也使得它能够结合到Vav并介导提高胞内钙响应和JNK信号通路(4,5)。

1. Tedder, T.F. et al. (1997) Immunity 6, 107-18.
2. Buhl, A.M. and Cambier, J.C. (1999) J Immunol 162, 4438-46.
3. Fujimoto, M. et al. (2000) Immunity 13, 47-57.
4. O'Rourke, L.M. et al. (1998) Immunity 8, 635-45.
5. Sato, S. et al. (1997) Proc Natl Acad Sci U S A 94, 13158-62.

原厂资料：

Specificity / Sensitivity

CD19 Antibody detects endogenous levels of CD19 independent of its phosphorylated state.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around the carboxy-terminus of human CD19. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CD19 is a 95 kDa coreceptor, which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).

1. Tedder, T.F. et al. (1997) Immunity 6, 107-18.
2. Buhl, A.M. and Cambier, J.C. (1999) J Immunol 162, 4438-46.
3. Fujimoto, M. et al. (2000) Immunity 13, 47-57.
4. O'Rourke, L.M. et al. (1998) Immunity 8, 635-45.
5. Sato, S. et al. (1997) Proc Natl Acad Sci U S A 94, 13158-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.