CD34 (ICO115) Mouse mAb

• 产品编号：CST-3569S      品牌：CST       原厂货号：3569S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CD34 (ICO115) Mouse mAb 能够检测内源水平CD34蛋白总体水平。此单克隆抗体是通过慢性粒细胞白血病患者的白血病细胞免疫 BALB/c小鼠而获得。CD34是糖基化的磷I类跨膜蛋白，在造血干/前体细胞，血管内皮细胞和一些成纤维细胞(1)。CD34的表达作为造血干细胞的标志已经应用很长一段时间了。CD34+造血干细胞在细胞因子和生长因子的刺激下扩展和分化成为淋巴造血谱系，同时在分化的过程中丢失CD34的表达。然而，最近几个实验室的研究与此论点冲突(2)。CD34的胞外区域与CD43同源,CD43参与了细胞-细胞的粘连作用,CD34的作用是作为细胞粘连的负调控因子(3)CD34 与CrkL（而不是CrkII）相互结合作为PKC的底物, PKC的激活与表面CD34的表达相偶联(1,4)。

1. Felschow, D. M. et al. (2001) Blood 97, 3768-3775.
2. Sato, T. et al. (1999) Blood 94, 2548-2554.
3. Drew, E. et al. (2005) Immunity 22, 43-57.
4. Fackler, M. J. et al. (1992) J. Biol. Chem. 267, 17540-17546.

原厂资料：

Specificity / Sensitivity

CD34 (ICO115) Mouse mAb detects endogenous levels of total CD34 protein.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with blast cells of a chronic myeloid leukemia patient.

Background

CD34 is a type I transmembrane glycophosphoprotein expressed by hematopoietic stem/progenitor cells, vascular endothelium and some fibroblasts (1). CD34 expression has been the hallmark used to identify hematopoietic stem cells for many years. CD34+ hematopoietic stem cells expand and differentiate into all the lymphohematopoietic lineages upon cytokine or growth factor stimulation and lose CD34 expression upon differentiation. However, recent studies performed in various laboratories conflict with that convention (2). The extracellular domain of CD34 is homologous to CD43, a protein involved in cell-cell adhesion, and CD34 has been shown to function as a negative regulator of cell adhesion (3). CD34 associates with CrkL but not CrkII, is a substrate for PKC, and activation of PKC is coupled with surface expression of CD34 (1,4).

1. Felschow, D. M. et al. (2001) Blood 97, 3768-3775.
2. Sato, T. et al. (1999) Blood 94, 2548-2554.
3. Drew, E. et al. (2005) Immunity 22, 43-57.
4. Fackler, M. J. et al. (1992) J. Biol. Chem. 267, 17540-17546.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.