TGM2 (D11A6) XP? Rabbit mAb

• 产品编号：CST-3557S      品牌：CST       原厂货号：3557S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TGM2 (D11A6) XP兔单克隆抗体识别内源性的全部TGM2蛋白。该单克隆抗体用与人类TGM2蛋白中Lys598位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。转谷氨酰胺酶2（TGM2）是一种钙依赖性酶,可以通过催化在赖氨酸和谷氨酸残基间形成化学键而交联细胞质内和细胞外基质中的蛋白（1）。这种双功能酶也具有内在的GTP酶活性，有报道认为转谷氨酰胺酶活性的调控可能是通过一个G-蛋白偶联受体信号通路完成的（2）。TGM2交联后的肽，可帮助调节细胞骨架结构，细胞迁移，细胞凋亡和细胞外基质的粘附。此外，这种酶在伤口愈合的免疫反应中起着重要的作用（3）。TGM2在体外可表现出激酶活性，可将胰岛素样生长因子结合蛋白3(IGFBP-3)作为一个可能的底物（4）。这种广泛表达的蛋白定位于细胞质和细胞核，但也已经从细胞表面和细胞外基质中分离得到（综述5）。因为它能和许多不同的底物相互作用，也因为其在损伤反应中的作用，TGM2已与一些人类疾病病理相关联。长期以来，人们一直认为TGM2是乳糜泻病的主要自身抗原（6）；TGM2表达或活性改变可能与阿尔茨海默病、亨廷顿病，动脉硬化，糖尿病和多种癌症有关（综述7）。

1. Griffin, M. et al. (2002) Biochem J 368, 377-96.
2. Jeon, J.H. et al. (2002) Biochem Biophys Res Commun 294, 818-22.
3. Fesus, L. and Piacentini, M. (2002) Trends Biochem Sci 27, 534-9.
4. Mishra, S. and Murphy, L.J. (2004) J Biol Chem 279, 23863-8.
5. Robinson, N.J. et al. (2007) Biol Reprod 77, 648-57.
6. Dieterich, W. et al. (1997) Nat Med 3, 797-801.
7. Facchiano, F. et al. (2006) Front Biosci 11, 1758-73.

原厂资料：

Specificity / Sensitivity

TGM2 (D11A6) XP® Rabbit mAb detects endogenous levels of total TGM2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys598 of human TGM2.

Background

Transglutaminase 2 (TGM2) is a calcium-dependent enzyme that cross-links both cytosolic and extracellular matrix proteins by catalyzing the formation of bonds between lysine and glutamine residues (1). This bifunctional enzyme also has intrinsic GTPase activity, and it has been suggested that regulation of the transamidase activity might be regulated through a G-protein coupled receptor-signaling pathway (2). In cross-linking peptides, TGM2 helps to regulate cytoskeletal structure, cell migration, apoptosis and cell-matrix adhesion. In addition, the enzyme plays an important role in wound healing and the immune response (3). TGM2 has exhibited kinase activity in vitro, with insulin-like growth factor-binding protein-3 (IGFBP-3) as one possible substrate (4). This widely expressed protein is localized to the cytosol and nucleus, but has also been isolated from the cell surface and extracellular matrix (reviewed in 5). Because of its interaction with a number of different substrates, and its role in the response to injury, TGM2 has been associated with the pathology of a number of human disorders. It has long been recognized as the major autoantigen in celiac disease (6); altered TGM2 expression or activity may be associated with Alzheimer disease, Huntington disease, arteriosclerosis, diabetes, and numerous forms of cancer (reviewed in 7).

1. Griffin, M. et al. (2002) Biochem J 368, 377-96.
2. Jeon, J.H. et al. (2002) Biochem Biophys Res Commun 294, 818-22.
3. Fesus, L. and Piacentini, M. (2002) Trends Biochem Sci 27, 534-9.
4. Mishra, S. and Murphy, L.J. (2004) J Biol Chem 279, 23863-8.
5. Robinson, N.J. et al. (2007) Biol Reprod 77, 648-57.
6. Dieterich, W. et al. (1997) Nat Med 3, 797-801.
7. Facchiano, F. et al. (2006) Front Biosci 11, 1758-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.