Mer (D21F11) XP? Rabbit mAb (Sepharose^? Bead Conjugate)

• 产品编号：CST-3551S      品牌：CST       原厂货号：3551S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Mer (D21F11) XP®兔单抗 (琼脂糖珠结合)能够检测内源性水平的总Mer蛋白。该单克隆抗体通过用合成肽免疫动物制备，该合成肽是人Mer蛋白组氨酸（925位）附近的残基。Cell Signaling Technology公司的抗体通过与主要的氨基基团共价结合而固定到N-羟基琥珀酰亚胺（NHS）活化的琼脂糖珠。Mer (D21F11) XP® 兔单抗 (琼脂糖珠结合)对于Mer的免疫沉淀是有用的。该抗体预期可以与非结合的Mer (D21F11) XP® 兔单抗#4319表现出同一物种的交叉反应。Mer和Axl、Tyro-3一样属于酪氨酸激酶受体家族。该家族的特征是有一个共同的N-CAM (神经牵粘分子)相关的胞外区和一个共同的配体GAS6 (生长休止特异蛋白6)。由于不同细胞中Mer蛋白有不同的糖基化修饰，所以其表观分子量在170-210 kDa之间。与配体结合或通过NCAM样基序亲同种抗原的细胞间相互作用后，Mer蛋白能够发生二聚化和自磷酸化激活（1）。活化的Mer下游信号通路组成包括PI3K激酶、 PLCγ 和 MAPK激酶 (2)。该家族成员往往参与转录调控并行使调控细胞粘附、迁移、吞噬和存活等功能（3）。Mer调控巨噬细胞活化、促进对凋亡细胞的吞噬并支持体内血小板凝集和血栓的稳定（4）。Mer过表达可能与白血病发生有关，而且有可能成为白血病和淋巴癌生物治疗的一个有效靶点（5）。

1. Ling, L. et al. (1996) J Biol Chem 271, 18355-62.
2. Ling, L. and Kung, H.J. (1995) Mol Cell Biol 15, 6582-92.
3. Hafizi, S. and Dahlbäck, B. (2006) Cytokine Growth Factor Rev 17, 295-304.
4. Sather, S. et al. (2007) Blood 109, 1026-33.
5. Keating, A.K. et al. (2006) Oncogene 25, 6092-100.

原厂资料：

Specificity / Sensitivity

Mer (D21F11) XP® Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total Mer protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His925 of human Mer protein.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Mer (D21F11) XP® Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of Mer. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Mer (D21F11) XP® Rabbit mAb #4319.

Background

Mer tyrosine kinase belongs to a receptor tyrosine kinase family with Axl and Tyro3. This family is characterized by a common NCAM (neural adhesion molecule)-related extracellular domain and a common ligand, GAS6 (growth arrest-specific protein 6). Mer protein has an apparent molecular weight of 170-210 kDa due to different glycosylation patterns generated in different cell types. Mer can be activated by dimerization and autophosphorylation through ligand binding or homophilic cell-cell interaction mediated by its NCAM-like motif (1). The downstream signaling components of activated Mer include PI3 kinase, PLCγ, and MAP kinase (2). Family members are prone to transcriptional regulation and carry out diverse functions including the regulation of cell adhesion, migration, phagocytosis, and survival (3). Mer regulates macrophage activation, promotes apoptotic cell engulfment, and supports platelet aggregation and clot stability in vivo (4). Investigators ahve found that overexpression of Mer may play a cooperative role in leukemogenesis and may be an effective target for biologically based leukemia/lymphoma therapy (5).

1. Ling, L. et al. (1996) J Biol Chem 271, 18355-62.
2. Ling, L. and Kung, H.J. (1995) Mol Cell Biol 15, 6582-92.
3. Hafizi, S. and Dahlbäck, B. (2006) Cytokine Growth Factor Rev 17, 295-304.
4. Sather, S. et al. (2007) Blood 109, 1026-33.
5. Keating, A.K. et al. (2006) Oncogene 25, 6092-100.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.