PIAS1 (D33A7) XP? Rabbit mAb

• 产品编号：CST-3550S      品牌：CST       原厂货号：3550S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PIAS1 (D33A7) XP® Rabbit mAb能够检测内源PIAS1蛋白总体水平。此单克隆抗体是通过合成人源对应的PIAS1 Ser550位点周围的肽段来免疫动物而获得。PIAS (激活的Stat的蛋白抑制剂)蛋白, 包括PIAS1, PIAS3, PIASx 和 PIASy,他们最初被鉴定是通过他们与Stat转录因子的相互作用(1,2)。PIAS1, PIAS3 和 PIASx分别与Stat1, Stat3 和 Stat4相互作用并抑制他们(1-3)。PIAS1的缺失导致了对干扰素诱导的基因的抑制并增强了对感染的抵抗保护(4)。PIAS家族的成员包含一个保守的RING区域，此区域的功能类似于 SUMO (小泛素相关修饰)连接酶, 偶联SUMO结合酶Ubc9及其底物蛋白(5,6)。大量的研究表明PIAS家族的成员能够通过不同的机制调控转录因子的活性,包括NF-κB (7,8), c-Jun, p53 (5,9), Oct-4 (11)和 Smads (11,12)。PIAS1的活性是通过磷酸化和精氨酸的甲基化。炎症刺激能够诱导IKK介导PIAS1在Ser90位点的磷酸化,这对其激活是必需的(13)。另外，在干扰素的处理下PRMT1诱导PIAS1在Arg303的甲基化，这与其抑制Stat1活性相关(14)。

1. Liu, B. et al. (1998) Proc Natl Acad Sci USA 95, 10626-31.
2. Chung, C.D. et al. (1997) Science 278, 1803-5.
3. Arora, T. et al. (2003) J Biol Chem 278, 21327-30.
4. Liu, B. et al. (2004) Nat Immunol 5, 891-8.
5. Schmidt, D. and Müller, S. (2002) Proc Natl Acad Sci USA 99, 2872-7.
6. Kotaja, N. et al. (2002) Mol Cell Biol 22, 5222-34.
7. Liu, B. et al. (2005) Mol Cell Biol 25, 1113-23.
8. Tahk, S. et al. (2007) Proc Natl Acad Sci USA 104, 11643-8.
9. Bischof, O. et al. (2006) Mol Cell 22, 783-94.
10. Tolkunova, E. et al. (2007) J Mol Biol 374, 1200-12.
11. Long, J. et al. (2004) Proc Natl Acad Sci USA 101, 99-104.
12. Murdoch, R.N. and Edwards, T. (1992) Biochem Int 28, 1029-37.
13. Liu, B. et al. (2007) Cell 129, 903-14.
14. Weber, S. et al. (2009) Genes Dev 23, 118-32.

原厂资料：

Specificity / Sensitivity

PIAS1 (D33A7) XP® Rabbit mAb detects endogenous levels of total PIAS1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser550 of human PIAS1.

Background

The PIAS (protein inhibitor of activated Stat) proteins, which include PIAS1, PIAS3, PIASx, and PIASy, were originally characterized based on their interaction with the Stat family of transcription factors (1,2). PIAS1, PIAS3, and PIASx interact with and repress Stat1, Stat3, and Stat4, respectively (1-3). Deletion of PIAS1 leads to inhibition of interferon-inducible genes and increased protection against infection (4). The PIAS family contains a conserved RING domain that has been linked to a function as a SUMO (small ubiquitin-related modifier) ligase, coupling the SUMO conjugating enzyme Ubc9 with its substrate proteins (5,6). Numerous studies have now shown that PIAS family members can regulate the activity of transcription factors through distinct mechanisms, including NF-κB (7,8), c-Jun, p53 (5,9), Oct-4 (11), and Smads (11,12). The activity of PIAS1 is regulated by both phosphorylation and arginine methylation. Inflammatory stimuli can induce IKK-mediated phosphorylation of PIAS1 at Ser90, which is required for its activity (13). In addition PRMT1 induces arginine methylation of PIAS1 at Arg303 following interferon treatment and is associated with its repressive activity on Stat1 (14).

1. Liu, B. et al. (1998) Proc Natl Acad Sci USA 95, 10626-31.
2. Chung, C.D. et al. (1997) Science 278, 1803-5.
3. Arora, T. et al. (2003) J Biol Chem 278, 21327-30.
4. Liu, B. et al. (2004) Nat Immunol 5, 891-8.
5. Schmidt, D. and Müller, S. (2002) Proc Natl Acad Sci USA 99, 2872-7.
6. Kotaja, N. et al. (2002) Mol Cell Biol 22, 5222-34.
7. Liu, B. et al. (2005) Mol Cell Biol 25, 1113-23.
8. Tahk, S. et al. (2007) Proc Natl Acad Sci USA 104, 11643-8.
9. Bischof, O. et al. (2006) Mol Cell 22, 783-94.
10. Tolkunova, E. et al. (2007) J Mol Biol 374, 1200-12.
11. Long, J. et al. (2004) Proc Natl Acad Sci USA 101, 99-104.
12. Murdoch, R.N. and Edwards, T. (1992) Biochem Int 28, 1029-37.
13. Liu, B. et al. (2007) Cell 129, 903-14.
14. Weber, S. et al. (2009) Genes Dev 23, 118-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.