Phospho-GSK-3β (Thr390) Antibody

• 产品编号：CST-3548S      品牌：CST       原厂货号：3548S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-GSK-3β (Thr390) Antibody可以检测出在Thr390位点磷酸化的内源性总GSK-3β蛋白水平。多克隆抗体是采用合成的与人源GSK-3β蛋白Thr390位点周围序列相对应的磷酸肽段免疫动物生产的，抗体采用蛋白A和肽亲和层析法纯化。糖原合酶激酶-3（GSK-3）最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶（1）。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶，可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子，其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制（2,3）。GSK-3不仅参与盘基网柄菌细胞命运的调控，而且是果蝇，爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分（4）。GSK-3还参与调节cyclin D1 蛋白降解和亚细胞定位（5）。

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
6. Thornton, T.M. et al. (2008) Science 320, 667-70.
7. Daub, H. et al. (2008) Mol Cell 31, 438-48.
8. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
9. Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
10. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci USA 101, 12130-5.
11. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

原厂资料：

Specificity / Sensitivity

Phospho-GSK-3β (Thr390) Antibody detects endogenous levels of human GSK-3β protein only when phosphorylated at Thr390.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr390 of human GSK-3β. Antibodies are purified by peptide affinity chromatography.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Phosphorylation of Thr390 on GSK-3β was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery (6). The phosphorylation of this site was also reported by several other labs using phosphoproteomic analysis on mitotic cell extracts (7-10).

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
6. Thornton, T.M. et al. (2008) Science 320, 667-70.
7. Daub, H. et al. (2008) Mol Cell 31, 438-48.
8. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
9. Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
10. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci USA 101, 12130-5.
11. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.