Arf6 Antibody

• 产品编号：CST-3546S      品牌：CST       原厂货号：3546S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Arf6 Antibody识别内源性的Arf6总蛋白。该多克隆抗体用与人Arf6中Gly165位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。ADP-核糖基化因子（Arf）蛋白是低分子量的GTP结合蛋白，属于Ras GTP酶超家族（1）。Arf蛋白根据氨基酸序列和结构相似性分为三个不同类型，Arf6目前是第III类中的唯一成员。Arf6主要定位于细胞膜和内体（1，2）。这个小GTP酶与PIP5K、PLD和Rac1相互作用，它们都是与脂肪代谢和肌动蛋白调节中重要的蛋白。Arf6的功能与其在GDP和GTP结合状态的循环有关，由结合的GAP和GEF因子调节（3，4）。质膜结合的Arf6在细胞膜转运的很多步骤中扮演重要功能，包括以clathrin依赖或非依赖的方式调节质膜受体内化、内体循环和远端激动蛋白重组和重塑（5，6）。

1. Gillingham, A.K. and Munro, S. (2007) Annu Rev Cell Dev Biol 23, 579-611.
2. Donaldson, J.G. and Honda, A. (2005) Biochem Soc Trans 33, 639-42.
3. Sakagami, H. (2008) Tohoku J Exp Med 214, 191-8.
4. Casanova, J.E. (2007) Traffic 8, 1476-85.
5. D'Souza-Schorey, C. and Chavrier, P. (2006) Nat Rev Mol Cell Biol 7, 347-58.
6. Donaldson, J.G. (2003) J Biol Chem 278, 41573-6.

原厂资料：

Specificity / Sensitivity

Arf6 Antibody detects endogenous levels of total Arf6 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly165 of human Arf6 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

ADP-ribosylation factor (Arf) proteins are low molecular weight GTP binding proteins that belong to the Ras GTPase superfamily (1). Arf proteins are grouped into three distinct classes based on amino acid sequence and structural similarity, with Arf6 as the single class III protein to date. Arf6 is localized mainly to the plasma membrane and endosomes (1,2). This small GTPase interacts with PIP5K, PLD and Rac1, proteins important in lipid metabolism and actin regulation. Arf6 function depends upon its cycling between GDP- and GTP-bound states, which is regulated by associated GAP and GEF factors (3,4). Plasma membrane-associated Arf6 appears to play several functions during the many steps of membrane trafficking, including regulating membrane receptor internalization in both clathrin-dependent and independent pathways, endosomal recycling, and proximal actin reorganization and remodeling (5,6).

1. Gillingham, A.K. and Munro, S. (2007) Annu Rev Cell Dev Biol 23, 579-611.
2. Donaldson, J.G. and Honda, A. (2005) Biochem Soc Trans 33, 639-42.
3. Sakagami, H. (2008) Tohoku J Exp Med 214, 191-8.
4. Casanova, J.E. (2007) Traffic 8, 1476-85.
5. D'Souza-Schorey, C. and Chavrier, P. (2006) Nat Rev Mol Cell Biol 7, 347-58.
6. Donaldson, J.G. (2003) J Biol Chem 278, 41573-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.