Nod1 Antibody

• 产品编号：CST-3545S      品牌：CST       原厂货号：3545S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Nod1 抗体能够检测内源水平Nod1蛋白。此多克隆抗体是通过合成对应的Nod1 C-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。Nod1/CARD4 是与Apaf-1和植物抗药蛋白结构上相关的细胞质蛋白，它参与到凋亡和对某些病原性细菌的炎症应答过程中(1-3)。蛋白包含一个N-末端级联反应招募区域(CARD)连接到核酸结合区域(NBD; 也叫做NOD区域)，紧随其后的是C-末端的富含亮氨酸的重复(LRR)区域 (1)。与Apaf-1相似, Nod1 通过CARD/NBD依赖的方式激活caspase-9而诱导凋亡(1)。Nod1最主要的作用是感知特定病原性微生物并引起炎症应答包括NF-κB 和 JNK信号通路的激活(4-6)。Nod1的LRR区域似乎参与到了微生物成分的识别，CARD 区域诱导了NF-κB的激活协同于含CARD区域的激酶RICK/RIP2/CARDIAK (1,5,6)。Nod1基因的突变增加了哮喘的敏感性(7)和炎症性肠病(8)。

1. Inohara, N. et al. (1999) J. Biol. Chem. 274, 14560-14567.
2. Inohara, N. and Nuñez, G. (2003) Nat. Rev. Immunol. 3, 371-382.
3. Fritz, J.H. et al. (2006) Nat .Immunol. 7, 1250-1257.
4. Girardin, S.E. et al. (2001) EMBO Rep. 2, 736-742.
5. Inohara, N. et al. (2001) J. Biol. Chem. 276, 2551-2554.
6. Inohara, N. et al. (2000) J. Biol. Chem. 275, 27823-27831.
7. Hysi, P. et al. (2005) Hum. Mol. Genet. 14, 935-941.
8. McGovern, D.P. et al. (2005) Hum. Mol. Genet. 14, 1245-1250.

原厂资料：

Specificity / Sensitivity

Nod1 Antibody detects endogenous levels of Nod1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of Nod1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Nod1/CARD4 is a cytosolic protein structually related to Apaf-1 and plant drug-resistance proteins that has been implicated in apoptosis and inflammatory responses to certain pathogenic bacteria (1-3). It contains an amino-terminal caspase recruitment domain (CARD) that is linked to a central nucleotide-binding domain (NBD; also known as a NOD domain) and is followed by carboxy-terminal leucine-rich repeats (LRR) (1). Like Apaf-1, Nod1 induces apoptosis by a CARD/NBD-dependent activation of caspase-9 (1). The primary function of Nod1 is thought to be as a sensor for certain pathogenic microbes and triggering inflammatory responses including the activation of NF-κB and JNK pathways (4-6). The LRR of Nod1 appears to be involved in recognition of microbial components and the CARD domain induces NF-κB activation in cooperation with the CARD containing kinase, RICK/RIP2/CARDIAK (1,5,6). Mutations in Nod1 have been linked increased susceptibility to asthma (7) and inflammatory bowel disease (8).

1. Inohara, N. et al. (1999) J. Biol. Chem. 274, 14560-14567.
2. Inohara, N. and Nuñez, G. (2003) Nat. Rev. Immunol. 3, 371-382.
3. Fritz, J.H. et al. (2006) Nat .Immunol. 7, 1250-1257.
4. Girardin, S.E. et al. (2001) EMBO Rep. 2, 736-742.
5. Inohara, N. et al. (2001) J. Biol. Chem. 276, 2551-2554.
6. Inohara, N. et al. (2000) J. Biol. Chem. 275, 27823-27831.
7. Hysi, P. et al. (2005) Hum. Mol. Genet. 14, 935-941.
8. McGovern, D.P. et al. (2005) Hum. Mol. Genet. 14, 1245-1250.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.