Phospho-NPM (Thr199) Antibody

• 产品编号：CST-3541S      品牌：CST       原厂货号：3541S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-NPM (Thr199) Antibody 能够检测内源性苏氨酸（199位）磷酸化的NPM蛋白。该多克隆抗体是由合成的人源的针对苏氨酸（199位）的磷酸化肽段免疫动物，利用蛋白A和多肽亲和层析技术生产的。核磷蛋白(NPM；又称B23、Numatrin或N038)是一种高丰度的核仁磷酸化蛋白。它拥有几个独特的细胞职能，包括核糖体组装和运输，胞质/胞核运输，DNA聚合酶α活性的调节，中心体复制和分子伴侣活性(1,2)。NPM的编码基因也因为和间变性淋巴瘤激酶(ALK)酪氨酸受体激酶的融合而广为人知。NPM部分通过提供一个二聚体结构域促进转化，最终激活融合激酶(3,4)。NPM与未复制的中心体结合，在中心体复制过程中是Cdk2-cyclin E的一个直接底物(4)。Cdk2-cyclin E磷酸化NPM苏氨酸199位后，NPM与中心体解聚，这是中心体复制起始的前提(5)。

1. Okuda, M. et al. (2000) Cell 103, 127-140.
2. Takemura, M. et al. (1999) J. Biochem. (Tokyo) 125, 904-909.
3. Morris, S.W. et al. (1994) Science 263, 1281-1284.
4. Bischof, D. et al. (1997) Mol. Cell. Biol. 17, 2312-2325.
5. Tokuyama, Y. et al. (2001) J. Biol. Chem. 276, 21529-21537.

原厂资料：

Specificity / Sensitivity

Phospho-NPM (Thr199) Antibody detects endogenous levels of NPM only when phosphorylated at threonine 199.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr199 of human NPM. Antibodies are purified by protein A and peptide affinity chromatography

Background

Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4).

NPM associates with unduplicated centrosomes and is a direct substrate of Cdk2-cyclin E in centrosome duplication (4). Upon phosphorylation at Thr199 by Cdk2-cyclin E, NPM dissociates from centrosomes, and this dissociation is a prerequisite step for centrosome to initiate duplication (5).

1. Okuda, M. et al. (2000) Cell 103, 127-140.
2. Takemura, M. et al. (1999) J. Biochem. (Tokyo) 125, 904-909.
3. Morris, S.W. et al. (1994) Science 263, 1281-1284.
4. Bischof, D. et al. (1997) Mol. Cell. Biol. 17, 2312-2325.
5. Tokuyama, Y. et al. (2001) J. Biol. Chem. 276, 21529-21537.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.