RalB Antibody

• 产品编号：CST-3523S      品牌：CST       原厂货号：3523S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RalB Antibody识别内源性RalB总蛋白。该抗体也可能与过表达的RalA蛋白发生相互作用。通过人工合成人源RalB蛋白Glu142位点相应的多肽片段去免疫动物从而制备出多克隆抗体。通过多肽亲和层析纯化抗体。RalA和RalB蛋白是小GTPases的Ras家族的成员，并且在蛋白质序列中是高度同源的。RalA和RalB蛋白的功能是有明显的重叠。通过结合到不同种的效应蛋白，RalA和RalB蛋白对于胞外分泌和膜运输作为重要的GTP感受器(1-3)。RalA蛋白对Ras相关的肿瘤生长是需要的(4)和RalB蛋白对肿瘤生存起着重要作用的(5)。除了肿瘤的形成，Ral蛋白也在癌症细胞迁移和转移性肿瘤入侵中起到重要作用(6,7)。

1. Li, G. et al. (2007) J Neurosci 27, 190-202.
2. van Dam, E.M. and Robinson, P.J. (2006) Int J Biochem Cell Biol 38, 1841-7.
3. Camonis, J.H. and White, M.A. (2005) Trends Cell Biol 15, 327-32.
4. Lim, K.H. et al. (2005) Cancer Cell 7, 533-45.
5. Chien, Y. et al. (2006) Cell 127, 157-70.
6. Oxford, G. et al. (2005) Cancer Res 65, 7111-20.
7. Lim, K.H. et al. (2006) Curr Biol 16, 2385-94.

原厂资料：

Specificity / Sensitivity

RalB Antibody detects endogenous levels of total RalB protein. The antibody might also react with overexpressed RalA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu142 of human RalB protein. Antibodies are purified by peptide affinity chromatography.

Background

RalA and RalB are members of the Ras family of small GTPases and are highly homologous in protein sequence. The functions of RalA and RalB are distinct yet overlapping. By binding to various effector proteins, RalA and RalB serve as important GTP sensors for exocytosis and membrane trafficking (1-3). RalA is required for Ras-related tumorigenesis (4) and RalB is important for tumor survival (5). In addition to tumor formation, Ral proteins also play a role in cancer cell migration and metastatic tumor invasion (6,7).

1. Li, G. et al. (2007) J Neurosci 27, 190-202.
2. van Dam, E.M. and Robinson, P.J. (2006) Int J Biochem Cell Biol 38, 1841-7.
3. Camonis, J.H. and White, M.A. (2005) Trends Cell Biol 15, 327-32.
4. Lim, K.H. et al. (2005) Cancer Cell 7, 533-45.
5. Chien, Y. et al. (2006) Cell 127, 157-70.
6. Oxford, G. et al. (2005) Cancer Res 65, 7111-20.
7. Lim, K.H. et al. (2006) Curr Biol 16, 2385-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.