Phospho-NPM (Ser4) (D19C1) XP? Rabbit mAb

• 产品编号：CST-3520P      品牌：Cell Signaling Technology       原厂货号：3520P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb 能够检测内源性丝氨酸（4位）磷酸化的NPM蛋白。该多克隆抗体是由合成的人源的针对NPM蛋白丝氨酸（4位点）的磷酸化肽段免疫动物，利用A蛋白和多肽亲和层析技术生产的。核磷蛋白(NPM；又称B23、Numatrin或N038)是一种高丰度的核仁磷酸化蛋白。它拥有几个独特的细胞职能，包括核糖体组装和运输，胞质/胞核运输，DNA聚合酶α活性的调节，中心体复制和分子伴侣活性(1,2)。NPM的编码基因也因为和间变性淋巴瘤激酶(ALK)酪氨酸受体激酶的融合而广为人知。NPM部分通过提供一个二聚体结构域促进转化，最终激活融合激酶(3,4)。

1. Okuda, M. et al. (2000) Cell 103, 127-140.
2. Takemura, M. et al. (1999) J. Biochem. (Tokyo) 125, 904-909.
3. Morris, S.W. et al. (1994) Science 263, 1281-1284.
4. Bischof, D. et al. (1997) Mol. Cell. Biol. 17, 2312-2325.

原厂资料：

Specificity / Sensitivity

Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb detects endogenous levels of NPM only when phosphorylated at Ser4.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser4 of human NPM. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4).

1. Okuda, M. et al. (2000) Cell 103, 127-140.
2. Takemura, M. et al. (1999) J. Biochem. (Tokyo) 125, 904-909.
3. Morris, S.W. et al. (1994) Science 263, 1281-1284.
4. Bischof, D. et al. (1997) Mol. Cell. Biol. 17, 2312-2325.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.