Brg1 (P680) Antibody

• 产品编号：CST-3514S      品牌：CST       原厂货号：3514S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Brg1 (P680) Antibody能够检测内源性Brg1总蛋白水平。通过合成的与人源Brg1蛋白Pro680位点周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。染色质结构的调整在转录激活和抑制的调节中是一个本质过程。至少两个从进化角度上有两个保守的过程进行修饰，ATP依赖的染色质改造蛋白干扰组蛋白和DNA联系，或者通过组蛋白尾部修饰包括甲基化和乙酰化。四类ATP依赖的组蛋白改造蛋白之一是SWI/SNF复合物，它的中心催化亚单位是Brg1或高度相关的蛋白hBRM(1)。SWI/SNF复合物包含不同的亚单位，但是它与Brg1或hBRM的关联仍然是不变的。研究显示SWI/SNF 复合物去调节基因激活、细胞生长、细胞周期和分化(1)。Brg1/hBRM已经显示通过提高糖皮质激素受体的转录激活去调节转录(2)。虽然通过与转录激活有关联，Brg1/hBRM也已经被发现在与转录抑制相关的复合物包括HDACs、Rb和Tif1β (3-5)。Brg1/hBRM在胚胎发育期间基因转录的调节中起着关键的作用。此外，Brg1/hBRM也作为一个肿瘤抑制因子的作用，并且Brg1在数个肿瘤细胞系中是突变的(6-8)。

1. Trotter, K.W. and Archer, T.K. (2008) Nucl Recept Signal 6, e004.
2. Trotter, K.W. and Archer, T.K. (2007) Mol Cell Endocrinol 265-266, 162-7.
3. Sif, S. et al. (2001) Genes Dev 15, 603-18.
4. Zhang, H.S. et al. (2000) Cell 101, 79-89.
5. Underhill, C. et al. (2000) J Biol Chem 275, 40463-70.
6. Magnani, L. and Cabot, R.A. (2009) Reproduction 137, 23-33.
7. Medina, P.P. et al. Epigenetics 3, 64-8.
8. Medina, P.P. et al. (2008) Hum Mutat 29, 617-22.

原厂资料：

Specificity / Sensitivity

Brg1 (P680) Antibody detects endogenous levels of Brg1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Pro680 of human Brg1. Antibodies are purified by Protein A and peptide affinity chromatography.

Background

The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression including with HDACs, Rb and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also play a role as a tumor suppressors and Brg1 is mutated in several tumor cell lines (6-8).

1. Trotter, K.W. and Archer, T.K. (2008) Nucl Recept Signal 6, e004.
2. Trotter, K.W. and Archer, T.K. (2007) Mol Cell Endocrinol 265-266, 162-7.
3. Sif, S. et al. (2001) Genes Dev 15, 603-18.
4. Zhang, H.S. et al. (2000) Cell 101, 79-89.
5. Underhill, C. et al. (2000) J Biol Chem 275, 40463-70.
6. Magnani, L. and Cabot, R.A. (2009) Reproduction 137, 23-33.
7. Medina, P.P. et al. Epigenetics 3, 64-8.
8. Medina, P.P. et al. (2008) Hum Mutat 29, 617-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.