Phospho-Connexin 43 (Ser368) Antibody

• 产品编号：CST-3511S      品牌：CST       原厂货号：3511S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化的 Connexin 43（丝氨酸368）仅仅在368位丝氨酸磷酸化时检测内源性Connexin 43的水平。抗体不与其他磷酸化Connexin 家族成员交叉反应。多克隆抗体由合成的磷酸化肽段免疫动物产生，该肽段与人connexin 43的368位丝氨酸附近残基对应。抗体由蛋白A和肽段亲和层析纯化得到。Connexin 43 (Cx43)是一类大的缝隙连接蛋白质类家族成员。连接蛋白类组装成一个六聚物，被运输到胞质膜产生一个半通道，这个半通道与附近细胞的半通道相联系，这样组成细胞与细胞的通道。这些通道汇集成簇形成缝隙接合部。缝隙接合部的交流在细胞生长调控和发育中很重要（1,2）。佛波醇酯类激活PKC后，Cx43 的368位丝氨酸被蛋白激酶C(PKC)磷酸化，这会减少细胞与细胞的交流（3）。Src能与Cx43相互作用并磷酸化Cx43，以改变缝隙连接的交流（4,5）。

1. Musil, L. S. et al. (1990) J. Cell. Biol. 111, 2077-2088.
2. Musil, L.S. and Goodenough, D.A. (1991) J. Cell. Biol. 115, 1357-1374.
3. Lampe, P. D. et al. (2000) J. Cell. Biol. 149, 1503-1512.
4. Giepmans, B. N. et al. (2001) J. Biol. Chem. 276, 8544-8549.
5. Lin, R. et al. (2001) J. Cell. Biol. 154, 815-827.

原厂资料：

Specificity / Sensitivity

Phospho-Connexin 43 (Ser368) Antibody detects endogenous levels of connexin 43 only when phosphorylated at serine 368. The antibody does not cross-react with other phosphorylated connexin family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser368 of human connexin 43. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (1,2). Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication (3). Src can interact with and phosphorylate Cx43 to alter gap junction communication (4,5).

1. Musil, L. S. et al. (1990) J. Cell. Biol. 111, 2077-2088.
2. Musil, L.S. and Goodenough, D.A. (1991) J. Cell. Biol. 115, 1357-1374.
3. Lampe, P. D. et al. (2000) J. Cell. Biol. 149, 1503-1512.
4. Giepmans, B. N. et al. (2001) J. Biol. Chem. 276, 8544-8549.
5. Lin, R. et al. (2001) J. Cell. Biol. 154, 815-827.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.