PDI (C81H6) Rabbit mAb

• 产品编号：CST-3501P      品牌：Cell Signaling Technology       原厂货号：3501P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PDI (C81H6) Rabbit mAb兔单抗识别内源性的PDI总蛋白。该单克隆抗体用与人类PDI蛋白氨基酸序列对应的人工合成肽段免疫动物而制成。在分泌蛋白合成过程中，其转位到内质网（ER）中进行翻译后修饰和正确折叠。为了形成其天然构象，很多分泌蛋白需要形成分子间或分子内二硫键（1）。这个过程称为氧化蛋白折叠。蛋白二硫键异构酶（PDI）催化这些二硫键的形成和异构（2）。对氧化折叠的研究提示分子氧可以氧化内质网蛋白质Ero1，Ero1反过来通过二硫键交换氧化PDI（3）。这些事件后就是PDI催化折叠中的蛋白质形成二硫键（3）。

1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-148.
2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32.
3. Tu, B.P. and Weissman, J.S. (2004) J. Cell Biol. 164, 341-346.

原厂资料：

Specificity / Sensitivity

PDI (C81H6) Rabbit mAb detects endogenous levels of total PDI protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PDI.

Background

During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).

1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-148.
2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32.
3. Tu, B.P. and Weissman, J.S. (2004) J. Cell Biol. 164, 341-346.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.