Beclin-1 (D40C5) Rabbit mAb

• 产品编号：CST-3495P      品牌：Cell Signaling Technology       原厂货号：3495P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Beclin-1 (D40C5) Rabbit mAb 兔单抗能检测内源性的Beclin-1 总蛋白。该单克隆抗体是通过用合成肽免疫动物制备的，该合成肽的序列与人Beclin-1蛋白的Thr72周围的残基相一致。自噬是自噬溶酶体降解蛋白质的一种分解代谢过程，由营养匮乏或神经退行性疾病引起（1）。Beclin-1是该过程中的关键蛋白之一，是哺乳动物中与酵母自噬蛋白Apg6/Vps30同源的基因（2）。Beclin-1可以补充由Apg6损失而造成的酵母自噬中的缺损，也可以在哺乳动物细胞中过表达时刺激细胞自噬（3）。哺乳动物Beclin-1最初是在酵母双杂交筛选中与Bcl-2相互作用的蛋白中分离出来的，能够与Bcl-2和Bcl-xL相互作用，而不能与Bax或Bak相互作用（4）。虽然Beclin-1普遍表达，但是研究发现，其在40%-75% 零散的人乳腺癌和卵巢癌中的等位基因都被删除了（5）。Beclin-1主要位于胞浆结构中，包括线粒体，Beclin-1的过表达会引起核染色和CRM1依赖的出核转运（6）。研究发现，Beclin-1 -/-小鼠在胚胎发育早期就会死亡，而Beclin-1 -/+小鼠则有较高的自发肿瘤发病率。来自null小鼠的干细胞显示出，自噬反应发生改变，而细胞凋亡反应则正常（7）。研究还发现，在体内，病毒感染神经元时，Beclin-1的过表达能针对Sindbis病毒诱导的疾病和神经元凋亡产生显著的保护作用（4）。

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Kametaka, S. et al. (1998) J Biol Chem 273, 22284-91.
3. Liang, X.H. et al. (1999) Nature 402, 672-6.
4. Liang, X.H. et al. (1998) J Virol 72, 8586-96.
5. Aita, V.M. et al. (1999) Genomics 59, 59-65.
6. Liang, X.H. et al. (2001) Cancer Res 61, 3443-9.
7. Yue, Z. et al. (2003) Proc Natl Acad Sci USA 100, 15077-82.

原厂资料：

Specificity / Sensitivity

Beclin-1 (D40C5) Rabbit mAb detects endogenous levels of total Beclin-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr72 of human Beclin-1.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). It is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Beclin-1 -/- mice die early in embryogenesis and Beclin-1 -/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response although responses to apoptosis appeared normal (7). Overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Kametaka, S. et al. (1998) J Biol Chem 273, 22284-91.
3. Liang, X.H. et al. (1999) Nature 402, 672-6.
4. Liang, X.H. et al. (1998) J Virol 72, 8586-96.
5. Aita, V.M. et al. (1999) Genomics 59, 59-65.
6. Liang, X.H. et al. (2001) Cancer Res 61, 3443-9.
7. Yue, Z. et al. (2003) Proc Natl Acad Sci USA 100, 15077-82.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.