AQP2 Antibody

• 产品编号：CST-3487S      品牌：CST       原厂货号：3487S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AQP2 Antibody用于检测内源性AQP2总蛋白水平。多克隆抗体通过用对应于人源AQP2蛋白Gln263序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化制得。水孔蛋白2（AQP2）是水的转运蛋白，它在肾小管中形成水的通道，在机体的水平衡调节中发挥重要作用。水孔蛋白家族成员都是多次跨膜蛋白，在质膜成形成同源四聚体水通道，促进水分子通过质膜。目前至少发现了13种水孔蛋白(从AQP0到AQP12)，这组小的疏水蛋白在多种生物学过程中发挥作用，包括尿的形成，细胞运动，生殖，细胞连结的形成以及总的水平衡（2）。AQP2四聚体在肾脏中形成水通道，促进水分子转动和分泌（3）。该蛋白定位于质膜，受到内分泌系统的调控。垂体后叶激素精氨酸抗利尿素（AVP）和ADH通过促进AQP2的磷酸化及伴随的胞吐作用来调节细胞的水渗透性（1,4）。AQP2 的突变会导致一种罕见的糖尿病，叫做肾发性糖尿病尿崩症。这种常染色体显性遗传疾病主要特点是肾小管异常的水重吸收，一部分原因是由于AQP2蛋白的错误定位或功能失常（5)。

1. Takata, K. et al. (2008) Histochem Cell Biol 130, 197-209.
2. Verkman, A.S. and Mitra, A.K. (2000) Am J Physiol Renal Physiol 278, F13-28.
3. Kamsteeg, E.J. et al. (2000) J Cell Biol 151, 919-30.
4. van Balkom, B.W. et al. (2002) J Biol Chem 277, 41473-9.
5. Loonen, A.J. et al. (2008) Semin Nephrol 28, 252-65.

原厂资料：

Specificity / Sensitivity

AQP2 Antibody detects endogenous levels of total AQP2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln263 of human AQP2. Antibodies are purified by peptide affinity chromatography.

Background

Aquaporin 2 (AQP2) is a water transport protein that forms water channels in kidney tubules and plays a predominant role in controlling organism water homeostasis (1). Members of the aquaporin family are multiple pass transmembrane proteins that form homotetramers to facilitate the flow of water across the plasma membrane. At least thirteen aquaporins have been indentified to date (AQP0 through AQP12) and together this family of small, hydrophobic proteins plays a role in an array of biological processes that include urine formation, cell motility, fertilization, cell junction formation and regulation of overall water homeostasis (2). AQP2 tetramers form water channels that facilitate water transport and excretion in the kidney (3). This transport protein is localized to the plasma membrane is response to endocrine signaling. Posterior pituitary hormones arginine vasopressin (AVP) and ADH regulate osmotic water cell permeability by triggering phosphorylation and subsequent exocytosis of AQP2 (1,4). Mutations in the corresponding AQP2 gene cause a rare form of diabetes known as nephrogenic diabetes insipidus. This autosomal dominant disorder is characterized by abnormal water reabsorption by kidney tubules due, in part, to either nonfunctional or mislocalized AQP2 protein (5).

1. Takata, K. et al. (2008) Histochem Cell Biol 130, 197-209.
2. Verkman, A.S. and Mitra, A.K. (2000) Am J Physiol Renal Physiol 278, F13-28.
3. Kamsteeg, E.J. et al. (2000) J Cell Biol 151, 919-30.
4. van Balkom, B.W. et al. (2002) J Biol Chem 277, 41473-9.
5. Loonen, A.J. et al. (2008) Semin Nephrol 28, 252-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.