Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb

• 产品编号：CST-3482S      品牌：CST       原厂货号：3482S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb用于检测内源性在Ser428位磷酸化的LKB1蛋白水平。单克隆抗体通过用对应于人源LKB1蛋白Ser428位点（小鼠中是Ser431）序列的合成肽免疫动物制备而得。LKB1 (STK11)是一种丝氨酸/苏氨酸蛋白激酶，通过调控多种下游激酶信号通路来调控细胞结构，凋亡和能量平衡（1,2）。一个包括有LKB1，假定的激酶STRAD和MO25 脚手架蛋白和胞质蛋白复合体可以激活AMPK和AMPK相关激酶的活性（3）。AMPK是细胞能量平衡中的主要调控蛋白，控制多种下游的效应蛋白，在接受ATP浓度信号时发挥细胞生长和凋亡的调控作用（4）。在细胞内LKB1可以在多个位点被磷酸化，包括 Ser31/325/428 和 Thr189/336/363 (5)。LKB1的突变会引起Peutz-Jeghers综合征，该疾病是一种常染色体显性遗传疾病，主要特征是良性胃肠息肉和口、手、足上的黑色皮肤损伤（6）。LKB1其它的一些突变跟几种组织中弥散的肿瘤有关（7）。最近的研究表明，LKB1的Ser428被PKCζ磷酸化会影响它与AMPK结合并在Thr172位点磷酸化AMPK的能力（8），同时也会通过PTEN信号通路抑制Akt的表达来调控细胞凋亡。

1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.
8. Xie, Z. et al. (2006) J Biol Chem 281, 6366-75.
9. Song, P. et al. (2008) J Biol Chem 283, 12446-55.

原厂资料：

Specificity / Sensitivity

Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb detects endogenous levels of LKB1 when phosphorylated at Ser428.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser428 of human LKB1 (Ser431 of mouse).

Background

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, 
including human LKB1 at Ser31/325/428 and Thr189/336/363 (5). 
 Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).

Recent evidence suggests that phosphorylation at Ser428 by PKCζ influences the ability of LKB1 to bind and phosphorylate AMPK at Thr172 (8) as well as regulate apoptosis through PTEN signaling pathway suppression of Akt (9).

1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.
8. Xie, Z. et al. (2006) J Biol Chem 281, 6366-75.
9. Song, P. et al. (2008) J Biol Chem 283, 12446-55.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.