Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb

• 产品编号：CST-3476S      品牌：CST       原厂货号：3476S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化FGF Receptor (Tyr653/654) (55H2) 鼠单抗仅在Tyr 653/654被磷酸化后才能检测到转染表达的FGFR蛋白的存在。本抗体能与活化的PDGF和胰岛素/IGF-I receptors有轻微的交叉反应。单克隆抗体通过用多肽免疫动物得到，该磷酸化多肽是根据人的FGFR1蛋白Tyr653/654附近的氨基酸序列合成的，与 FGFR-2, -3 and -4上对应的序列相同。

成纤维细胞生长因子 (FGFs)通过细胞表面的酪氨酸激酶受体传递信号，对靶细胞产生促有丝分裂和血管生成的效应。已知FGF受体家族有四个成员：FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3和FGFR-4。每个受体都有一个胞外配体结合区、跨膜区和一个胞内激酶区（1）。在结合配体和二聚化之后，受体的特定的酪氨酸位点发生磷酸化（2）。在FGFR-1的羧基端有7个酪氨酸位点能被磷酸化：Tyr463, 583, 585, 653, 654, 730和 766。Tyr653 和Tyr654对激活的FGFR的催化活性十分重要，对信号传导也是必要的(3)。其它的磷酸化酪氨酸位点能够为下游信号分子，如Crk 和PLCγ，提供结合位点(4,5)。

1. Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
2. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
3. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
4. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.
5. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.

原厂资料：

Specificity / Sensitivity

Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb detects transfected levels of FGF receptors only when phosphorylated at tyrosines 653/654. The antibody cross-reacts slightly with activated PDGF and insulin/IGF-I receptors.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr653/654 of human FGF receptor-1. The corresponding sequence is identical in FGF receptor-2, -3 and -4.

Background

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3, and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).

1. Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
2. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
3. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
4. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.
5. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.