Perilipin (K117) Antibody

• 产品编号：CST-3467S      品牌：CST       原厂货号：3467S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Perilipin (K117) Antibody用于检测内源性Perilipin总蛋白水平。多克隆抗体通过用对应于人源Perilipin蛋白Lys117位点序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化制得。甘油三酯作为人体首要的能量储备，主要以脂肪滴的形式存储于体内。Perilipin位于脂肪滴的外层，起着保护脂质，防止被脂酶裂解的作用(1-3)。许多证据表明，PKA通过磷酸化perifipin来调控脂解作用(1,2,4,5)。perifipin的磷酸化使脂肪滴的形态发生改变，将脂肪滴暴露于内源性的脂酶中(如激素敏感型的脂酶)(2)。因此，perilipin在脂质的储存中起着重要的作用(2,5)。

1. Greenberg, A.S. et al. (1991) J Biol Chem 266, 11341-6.
2. Brasaemle, D.L. (2007) J Lipid Res 48, 2547-59.
3. Ducharme, N.A. and Bickel, P.E. (2008) Endocrinology 149, 942-9.
4. Egan, J.J. et al. (1990) J Biol Chem 265, 18769-75.
5. Brasaemle, D.L. et al. (2009) Mol Cell Biochem 326, 15-21.

原厂资料：

Specificity / Sensitivity

Perilipin (K117) Antibody detects endogenous levels of total perilipin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Lys117 of human perilipin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5).

1. Greenberg, A.S. et al. (1991) J Biol Chem 266, 11341-6.
2. Brasaemle, D.L. (2007) J Lipid Res 48, 2547-59.
3. Ducharme, N.A. and Bickel, P.E. (2008) Endocrinology 149, 942-9.
4. Egan, J.J. et al. (1990) J Biol Chem 265, 18769-75.
5. Brasaemle, D.L. et al. (2009) Mol Cell Biochem 326, 15-21.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.