Phospho-FLT3 (Tyr591) Antibody

• 产品编号：CST-3461S      品牌：CST       原厂货号：3461S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化FLT3 (Tyr591) 抗体仅在Tyr591被磷酸化后才能检测到内源FLT3的存在。本抗体不与FLT家族其成员交叉反应，但可能与其它酪氨酸磷酸化的蛋白激酶发生交叉反应。（专利US Patent No. 7,183,385属于CST公司）多克隆抗体通过用多肽免疫动物得到，该磷酸化多肽是根据人的FLT3蛋白Tyr591附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。

FMS-相关的酪氨酸激酶3 (FLT3, 也称Flk2)是III型酪氨酸激酶受体家族的成员之一，其余成员包括c-Kit, PDGFR 和M-CSF 受体。FLT3表达在早期的造血前体细胞上，并支持造血系统的发育和分化（1,2）。FLT3结合其配体FL后激活，进而导致了酪氨酸酶自磷酸化和下游靶点酪氨酸磷酸化的级联反应（3）。FL刺激之后，FLT3就和PI3K激酶的p85亚基、 SHP2、 GRB2和Shc相关联（4-6）。Tyr589/591定位于FLT3的近膜区并对FLT3的酪氨酸激酶活性的调控有重要作用。20%的急性骨髓性白血病病人中发生FLT3内部串联重复 (ITDs)体细胞突变（7）。

1. Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48.
2. Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30.
3. Namikawa, R. et al. (1996) Stem Cells 14, 388-95.
4. Beslu, N. et al. (1996) J Biol Chem 271, 20075-81.
5. Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9.
6. Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80.
7. Mizuki, M. et al. (2000) Blood 96, 3907-14.

原厂资料：

Specificity / Sensitivity

Phospho-FLT3 (Tyr591) Antibody detects endogenous FLT3 only when phosphorylated at tyrosine 591. The antibody does not cross-react with other FLT family members. It may cross-react with some tyrosine phosphorylated proteins. 
 (US Patent No. 7,183,385 and foreign equivalents assigned to Cell Signaling Technology, Inc.)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr591 of human FLT3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).

1. Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48.
2. Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30.
3. Namikawa, R. et al. (1996) Stem Cells 14, 388-95.
4. Beslu, N. et al. (1996) J Biol Chem 271, 20075-81.
5. Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9.
6. Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80.
7. Mizuki, M. et al. (2000) Blood 96, 3907-14.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.