MeCP2 (D4F3) XP? Rabbit mAb

• 产品编号：CST-3456P      品牌：Cell Signaling Technology       原厂货号：3456P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MeCP2 (D4F3) XP® Rabbit mAb兔单抗能够检测内源性MeCP2的蛋白水平(A和B亚型)。该抗体不与MBD蛋白发生交叉反应。通过合成的与人源MeCP2蛋白羧基端相应多肽片段去免疫动物从而制备出此单克隆抗体。Methyl-CpG-binding protein 2 (MeCP2)是methyl-CpG-binding domain (MBD)蛋白家族的初始成员，该家族包含MBD1、MBD2、MBD3、MBD4、MBD5和MBD6 (1-3)。这些蛋白离开MBD3，通过招募多种辅助抑制因子，结合在双核苷酸5´-CG-3´的背景下甲基化的胞嘧啶残基去建立和维持转录失活的染色质区域(2)。MeCP2招募组蛋白去乙酰化酶HDAC1和HDAC2，以及DNA甲基转移酶DNMT1(4-6)。MBD1连接着转录沉默和DNA复制，并且与组蛋白甲基转移酶ESET和SUV39H1相互作用(7,8)。MBD2和MBD3共纯化后作为NuRD (nucleosome remodeling and histone de-acetylation)共抑制复合物的一部分，该复合物包好染色质重塑ATPase Mi-2、HDAC1和HDAC2 (9,10)。MBD5和MBD6蛋白最近被鉴定，并且很少知道关于它们的蛋白质相互作用。MBD蛋白与癌症和其它疾病有关联；MBD4蛋白最重要特征是DNA修复中的作用，并且MBD2已经联系着肠癌(11,12)。MeCP2基因突变可引起神经发育障碍患有Rett综合症 (13)。MeCP2蛋白水平在神经元中表达高，在那里它在多种突触进程中起着关键的作用(14)。在多种生理刺激反应下，MeCP2在Ser421位点磷酸化，并且能够调节控制着树突模式和脊柱形态发生的基因(14)。在个体中这个过程的紊乱并伴随着改变的MeCP2，在Rett综合征中可引起病理学的改变。

1. Clouaire, T. and Stancheva, I. (2008) Cell Mol Life Sci 65, 1509-22.
2. Hendrich, B. and Bird, A. (1998) Mol Cell Biol 18, 6538-47.
3. Roloff, T.C. et al. (2003) BMC Genomics 4, 1.
4. Nan, X. et al. (1998) Nature 393, 386-9.
5. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
6. Fuks, F. et al. (2003) J Biol Chem 278, 4035-40.
7. Sarraf, S.A. and Stancheva, I. (2004) Mol Cell 15, 595-605.
8. Fujita, N. et al. (2003) J Biol Chem 278, 24132-8.
9. Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
10. Wade, P.A. et al. (1999) Nat Genet 23, 62-6.
11. Hendrich, B. et al. (1999) Nature 401, 301-4.
12. Sansom, O.J. et al. (2003) Nat Genet 34, 145-7.
13. Miltenberger-Miltenyi, G. and Laccone, F. (2003) Hum Mutat 22, 107-15.
14. Zhou, Z. et al. (2006) Neuron 52, 255-69.

原厂资料：

Specificity / Sensitivity

MeCP2 (D4F3) XP® Rabbit mAb detects endogenous levels of MeCP2 (both isoforms A and B). This antibody does not cross-react with other MBD proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human MeCP2.

Background

Methyl-CpG-binding protein 2 (MeCP2) is the founding member of a family of methyl-CpG-binding domain (MBD) proteins that also includes MBD1, MBD2, MBD3, MBD4, MBD5 and MBD6 (1-3). Apart from MBD3, these proteins bind methylated cytosine residues in the context of the di-nucleotide 5´-CG-3´ to establish and maintain regions of transcriptionally inactive chromatin by recruiting a variety of co-repressor proteins (2). MeCP2 recruits histone deacetylases HDAC1 and HDAC2, and the DNA methyltransferase DNMT1 (4-6). MBD1 couples transcriptional silencing to DNA replication and interacts with the histone methyltransferases ESET and SUV39H1 (7,8). MBD2 and MBD3 co-purify as part of the NuRD (nucleosome remodeling and histone de-acetylation) co-repressor complex, which contains the chromatin remodeling ATPase Mi-2, HDAC1 and HDAC2 (9,10). MBD5 and MBD6 have recently been identified and little is known regarding their protein interactions. MBD proteins are associated with cancer and other diseases; MBD4 is best characterized for its role in DNA repair and MBD2 has been linked to intestinal cancer (11,12). Mutations in the MeCP2 gene cause the neurologic developmental disorder Rett Syndrome (13). MeCP2 protein levels are high in neurons, where it plays a critical role in multiple synaptic processes (14). In response to various physiological stimuli, MeCP2 is phosphorylated on Ser421 and regulates the expression of genes controlling dendritic patterning and spine morphogenesis (14). Disruption of this process in individuals with altered MeCP2 may cause the pathological changes seen in Rett Syndrome.

1. Clouaire, T. and Stancheva, I. (2008) Cell Mol Life Sci 65, 1509-22.
2. Hendrich, B. and Bird, A. (1998) Mol Cell Biol 18, 6538-47.
3. Roloff, T.C. et al. (2003) BMC Genomics 4, 1.
4. Nan, X. et al. (1998) Nature 393, 386-9.
5. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
6. Fuks, F. et al. (2003) J Biol Chem 278, 4035-40.
7. Sarraf, S.A. and Stancheva, I. (2004) Mol Cell 15, 595-605.
8. Fujita, N. et al. (2003) J Biol Chem 278, 24132-8.
9. Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
10. Wade, P.A. et al. (1999) Nat Genet 23, 62-6.
11. Hendrich, B. et al. (1999) Nature 401, 301-4.
12. Sansom, O.J. et al. (2003) Nat Genet 34, 145-7.
13. Miltenberger-Miltenyi, G. and Laccone, F. (2003) Hum Mutat 22, 107-15.
14. Zhou, Z. et al. (2006) Neuron 52, 255-69.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.