Notch3 (8G5) Rat mAb

• 产品编号：CST-3446S      品牌：CST       原厂货号：3446S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Notch3(8G5)大鼠单抗识别内源性的总Notch3蛋白，它识别Notch3全长蛋白(270 kDa)和胞外截短的片段, 胞外截短的片段含有短的胞外区域，跨膜结构域和胞内区域(90 kDa)。此单抗由融合蛋白免疫动物产生，该融合蛋白与人源Notch3蛋白胞内氨基酸残基序列一致。Notch1是一个在细胞发育和命运决定过程中发挥功能的跨膜蛋白（1）。在notch1成熟过程中，notch1分子细胞外结构域会被一个成对碱性氨基酸蛋白酶样的转化酶剪切（2）。在与配体如Delta1结合或是细胞外钙离子耗竭后，notch1的羧基端被释放并随后在Gly1743 和 Val1744之间被切开（3,4）。结果导致细胞内处于活化状态的notch1片段转运至细胞核内并转录激活。Notch3是Notch家族成员，加工过程与Notch1相似（5）。它主要表达在动脉平滑肌细胞（SMC）。在Notch3的胞外区域突变改变了半胱氨酸残基数量，这与大脑常染色体显性动脉病皮质下梗死和脑白质病（CADASIL）有关, （CADASIL）为一种导致成人中风痴呆的遗传血管病(6-8)。近来研究显示Notch3在多种癌症过表达(9-11)。

1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
5. Baron, M. (2003) Semin Cell Dev Biol 14, 113-9.
6. Kalimo, H. et al. (2002) Brain Pathol 12, 371-84.
7. Karlström, H. et al. (2002) Proc Natl Acad Sci USA 99, 17119-24.
8. Monet, M. et al. (2007) Hum Mol Genet 16, 982-92.
9. Park, J.T. et al. (2006) Cancer Res 66, 6312-8.
10. Gramantieri, L. et al. (2007) Liver Int 27, 997-1007.
11. Yamaguchi, N. et al. (2008) Cancer Res 68, 1881-8.

原厂资料：

Specificity / Sensitivity

Notch3 (8G5) Rat mAb detects endogenous levels of total notch3 protein. It recognizes the full-length (270 kDa) and the extracellular truncated fragment containing a short extracellular region, the transmembrane domain and the intracellular region (90 kDa).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a fusion protein corresponding to intracellular residues of notch3.

Background

Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.

Notch3 is a member of notch family that is processed in a similar way to notch1 (5). It is expressed primarily in arterial smooth muscle cells (SMC). Mutations altering the number of cysteine residues in the notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults (6-8). Recent studies indicate that notch3 is overexpressed in many types of cancers (9-11).

1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
5. Baron, M. (2003) Semin Cell Dev Biol 14, 113-9.
6. Kalimo, H. et al. (2002) Brain Pathol 12, 371-84.
7. Karlström, H. et al. (2002) Proc Natl Acad Sci USA 99, 17119-24.
8. Monet, M. et al. (2007) Hum Mol Genet 16, 982-92.
9. Park, J.T. et al. (2006) Cancer Res 66, 6312-8.
10. Gramantieri, L. et al. (2007) Liver Int 27, 997-1007.
11. Yamaguchi, N. et al. (2008) Cancer Res 68, 1881-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.