HA-Tag (6E2) Mouse mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-3444S      品牌：CST       原厂货号：3444S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

HA-Tag (6E2) Mouse mAb (Alexa Fluor ® 647 Conjugate)用来检测具有HA表位标签的重组蛋白。此抗体可以识别转染细胞中与氨基或羧基末端融合的HA-Tag。单克隆抗体是用含有流感血凝素表位(YPYDVPDYA的合成肽段肽段免疫动物生产的。此种抗体在最佳条件下结合了Alexa Fluor ® 647 荧光染料，其F/p为2-6。 The Alexa Fluor ® 647 染料可以被红光激发（比如 633 nm He-Ne 激光），与Alexa Fluor ® 647 染料结合的抗体可以产生明亮的远红荧光，最大峰值在665nm。此种抗体在最佳条件下结合了Alexa Fluor ® 647 荧光染料，已经过内部测试，可以应用于HA标签蛋白转染细胞的流式细胞术分析中。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。HA标签式从流感血凝素蛋白的一个表位演化而来的。已经作为常规标签被广泛应用于表达载体中（1）

Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.

原厂资料：

Specificity / Sensitivity

HA-Tag (6E2) Mouse mAb (Alexa Fluor® 647 Conjugate) detects recombinant proteins containing the HA epitope tag. The antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1).

Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.