Id2 (D39E8) Rabbit mAb

• 产品编号：CST-3431S      品牌：CST       原厂货号：3431S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Id2 (D39E8) Rabbit mAb兔单抗识别内源性的总Id2 蛋白。单抗由合成肽段免疫动物产生，该肽段与邻近人源Id2 蛋白的Asn97氨基酸残基序列一致。DNA结合蛋白抑制因子2(Id2) 是Id蛋白成员，属于螺旋-环-螺旋（HLH）蛋白家族。Id蛋白通过结合作用抑制了特异转录因子的二聚化以及DNA结合能力（1-3）。Id2与多种转录因子相互作用包括E蛋白（5），TCS(4),Pax（6）和肿瘤抑制因子Rb（1）。Id2已经显示对调控细胞分化，增殖，发育，肿瘤生成有重要作用（7-9）.在肿瘤细胞中，增加的Id2水平使Rb失活，导致细胞转化和癌症（10,11）。因此，Id2也是癌症治疗是一个有潜力的药物靶标（12）。

1. Iavarone, A. et al. (1994) Genes Dev 8, 1270-84.
2. Sun, X.H. et al. (1991) Mol Cell Biol 11, 5603-11.
3. Roberts, E.C. et al. (2001) Mol Cell Biol 21, 524-33.
4. Yates, P.R. et al. (1999) EMBO J 18, 968-76.
5. Engel, I. and Murre, C. (2001) Nat Rev Immunol 1, 193-9.
6. Benezra, R. et al. (2001) Oncogene 20, 8334-41.
7. Lasorella, A. et al. (2001) Oncogene 20, 8326-33.
8. Zebedee, Z. and Hara, E. (2001) Oncogene 20, 8317-25.
9. Gabellini, C. et al. (2006) Oncogene 25, 5326-32.
10. Perk, J. et al. (2005) Nat Rev Cancer 5, 603-14.
11. Gray, M.J. et al. (2008) Oncogene 27, 7192-200.

原厂资料：

Specificity / Sensitivity

Id2 (D39E8) Rabbit mAb detects endogenous levels of total Id2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn97 of human Id2 protein.

Background

Inhibitor of DNA-binding-2 (Id2) is a member of the Id proteins which belong to the helix-loop-helix (HLH) protein family. The Id protein functions by binding to specific transcription factors and preventing their dimerization and DNA binding (1-3). Id2 interacts with a wide variety of transcription factors including E proteins (5), TCS (4), Pax (6) and the tumor suppressor Rb (1). Id2 has been shown to be important in regulating cellular differentiation, proliferation, development and tumorgenesis (7-9). In tumor cells, increased levels of Id2 functionally inactivate Rb, leading to cellular transformation and cancer (10,11). Id2 is therefore a promising therapeutic target for treatment of cancer (12).

1. Iavarone, A. et al. (1994) Genes Dev 8, 1270-84.
2. Sun, X.H. et al. (1991) Mol Cell Biol 11, 5603-11.
3. Roberts, E.C. et al. (2001) Mol Cell Biol 21, 524-33.
4. Yates, P.R. et al. (1999) EMBO J 18, 968-76.
5. Engel, I. and Murre, C. (2001) Nat Rev Immunol 1, 193-9.
6. Benezra, R. et al. (2001) Oncogene 20, 8334-41.
7. Lasorella, A. et al. (2001) Oncogene 20, 8326-33.
8. Zebedee, Z. and Hara, E. (2001) Oncogene 20, 8317-25.
9. Gabellini, C. et al. (2006) Oncogene 25, 5326-32.
10. Perk, J. et al. (2005) Nat Rev Cancer 5, 603-14.
11. Gray, M.J. et al. (2008) Oncogene 27, 7192-200.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.