RhoC (D40E4) Rabbit mAb

• 产品编号：CST-3430P      品牌：Cell Signaling Technology       原厂货号：3430P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RhoC (D40E4) Rabbit mAb检测内源性RhoC总蛋白。该抗体不能识别相关蛋白RhoA或RhoB蛋白。通过人工合成人源RhoC蛋白羧基端周围相应的多肽片段去免疫动物从而制备出单克隆抗体。Rho family small GTPases包括Rho、Rac和cdc42，该家族作为分子开关，调节细胞进程例如细胞迁移、粘附、扩增和分化。它们通过鸟苷酸交换因子（guanine nucleotide exchange factor，GEF)被激活，这将催化已结合的GDP交换成GTP，然而通过GTPase activating proteins (GAPs)被抑制，这将催化GTP的水解成GDP。第三个调节水平通过Rho GDP dissociation inhibitor (RhoGDI)的计量结合力被提供(1)。RhoA、RhoB和RhoC是高度保守的，但是出现不同的生物功能。羧基端的修饰和在亚细胞上的定位差异允许这些蛋白回应和作为明显的信号分子(2,3)。

1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
2. Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
3. Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.

原厂资料：

Specificity / Sensitivity

RhoC (D40E4) Rabbit mAb detects endogenous levels of total RhoC protein. The antibody does not cross-react with RhoA or RhoB.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human RhoC.

Background

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).

1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
2. Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
3. Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.