Rad50 Antibody

• 产品编号：CST-3427P      品牌：Cell Signaling Technology       原厂货号：3427P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rad50 antibody 能够检测内源性Rad50总蛋白。.该多克隆抗体是由合成的人源的针对Rad50蛋白氨基末端的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。DNA修复蛋白Rad50是染色体结构维持家族(SMC)的一员，在细胞周期检验点信号和DNA损伤的双链断裂修复应答过程中发挥重要作用(1-4)。Rad50和Mre11及Nbs1组成一个蛋白复合体，当DNA损伤时被激活(3)。在正常的人类细胞中，MRN复合物能够拴住线性DNA分子，在DNA末端之间提供一个弹性链接(1)。细胞中的基因突变能够影响MRN复合物中的蛋白导致基因组不稳定和癌症的发生(2)。

1. de Jager, M. et al. (2001) Mol. Cell 8, 1129-35.
2. Elledge, S.J. (1996) Science 274, 1664-1672.
3. Lee, J.H. and Lim, D.S. (2006) FEBS J. 273, 1630-36.
4. Teng, S.C. et al. (2006) J Mol Histol 37, 293-9.
5. Gatei, M. et al. (2011) J Biol Chem 286, 31542-56.

原厂资料：

Specificity / Sensitivity

Rad50 antibody detects endogenous levels of total Rad50 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human Rad50. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes family (SMC) and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage (1-4). Rad50 forms a complex with Mre11 and Nbs1 that becomes activated in response to DNA damage (3). In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends (1). Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex (2).

1. de Jager, M. et al. (2001) Mol. Cell 8, 1129-35.
2. Elledge, S.J. (1996) Science 274, 1664-1672.
3. Lee, J.H. and Lim, D.S. (2006) FEBS J. 273, 1630-36.
4. Teng, S.C. et al. (2006) J Mol Histol 37, 293-9.
5. Gatei, M. et al. (2011) J Biol Chem 286, 31542-56.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.