Rabbit (DA1E) mAb IgG XP ® 同型对照不能直接用于其它已知抗原的检测。它的功能是作为兔IgG单克隆抗体的对照。Rabbit (DA1E) mAb IgG XP ® 同型对照#3900 (全分子)与羟基丁二酰亚胺活化的琼脂糖微珠上的主要氨基基团发生共价反应而固定。 Rabbit (DA1E) mAb IgG XP ® 同型对照(琼脂糖微珠结合)用来检测非特异性的免疫沉淀复合体。对照抗体用来检测因Fc受体结合或者蛋白与蛋白之间的相互作用引起的一抗的非特异性结合。
原厂资料:
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control is not directed against any known antigen. It functions as a control for rabbit IgG monoclonal antibodies.
Description
Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (whole molecule) from Cell Signaling Technology is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.
Background
Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.
注意事项:
Storage:Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.