IKKε (D61F9) XP? Rabbit mAb

• 产品编号：CST-3416P      品牌：Cell Signaling Technology       原厂货号：3416P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IKKε (D61F9) XP® Rabbit mAb 能够检测内源IKKε蛋白总体水平。单克隆抗体是通过合成鼠源对应的IKKε C-末端周围的肽段来免疫动物而获得。NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。大部分激活因素采用通常信号通路即磷酸化诱导并通过蛋白水解酶体介导降解IκB的方法激活NF-κB (3-7)。此通路中最重要的步骤是激活高分子量的IκB激酶（IKK）复合体，此复合体催化的反应中有三个紧密相关的IKK亚基参与完成，IKKα and IKKβ 作为激酶的催化单元，IKKγ作为调控单元(8,9)。IKK的激活依赖于特定位点的磷酸化：IKKβ活性环中的 Ser177和Ser181位点以及IKKα中的serine 176 和 180 位点，这些位点的磷酸化引起蛋白构象的变化从而激活激酶（10-13）。最近，IKKα和IKKβ的两个同源蛋白得到了鉴定，分别是IKKε (也叫IKK-i)和TBK-1 (也叫T2K 或 NAK), 激活这些激酶中的任何一个都能导致NF-κB的激活。IKKε在N-末端包含一个与IKKα或IKKβ有30%相似度的激酶区域。IKKε主要在免疫细胞中表达，并在免疫响应中发挥了特殊的作用(14-18)。

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Zandi, E. et al. (1997) Cell 91, 243-52.
9. Karin, M. (1999) Oncogene 18, 6867-74.
10. DiDonato, J.A. et al. (1997) Nature 388, 548-54.
11. Mercurio, F. et al. (1997) Science 278, 860-6.
12. Johnson, L.N. et al. (1996) Cell 85, 149-58.
13. Delhase, M. et al. (1999) Science 284, 309-13.
14. Shimada, T. et al. (1999) Int Immunol 11, 1357-62.
15. Peters, R.T. et al. (2000) Mol Cell 5, 513-22.
16. Tojima, Y. et al. (2000) Nature 404, 778-82.
17. Bonnard, M. et al. (2000) EMBO J 19, 4976-85.
18. Peters, R.T. and Maniatis, T. (2001) Biochim Biophys Acta 1471, M57-62.

原厂资料：

Specificity / Sensitivity

IKKε (D61F9) XP® Rabbit mAb detects endogenous levels of total IKKε protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of mouse IKKε.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13).

Recently, two homologs of IKKα and IKKβ have been described, called IKKε (also known as IKK-i) and TBK-1 (also known as T2K or NAK), and activation of either of these kinases results in NF-κB activation. IKKε contains the kinase domain in its amino terminus, which shares 30% identity to that of IKKα or IKKβ. IKKε is expressed mainly in immune cells, and may play a special role in the immune response (14-18).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Zandi, E. et al. (1997) Cell 91, 243-52.
9. Karin, M. (1999) Oncogene 18, 6867-74.
10. DiDonato, J.A. et al. (1997) Nature 388, 548-54.
11. Mercurio, F. et al. (1997) Science 278, 860-6.
12. Johnson, L.N. et al. (1996) Cell 85, 149-58.
13. Delhase, M. et al. (1999) Science 284, 309-13.
14. Shimada, T. et al. (1999) Int Immunol 11, 1357-62.
15. Peters, R.T. et al. (2000) Mol Cell 5, 513-22.
16. Tojima, Y. et al. (2000) Nature 404, 778-82.
17. Bonnard, M. et al. (2000) EMBO J 19, 4976-85.
18. Peters, R.T. and Maniatis, T. (2001) Biochim Biophys Acta 1471, M57-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.