Myc-Tag (9B11) Mouse mAb （琼脂糖微球结合）可以检测细胞中包含Myc标为标签的蛋白。此抗体在转染后哺乳动物细胞中可以识别Myc-Tag。单克隆抗体是采用与人类c-Myc (EQKLISEEDL)的410-419残基相对应的合成肽段免疫动物而生产的。此种抗体通过其主要的氨基基团交联羟基丁二酰亚胺活化的琼脂糖微珠， Myc-Tag (9B111) Mouse mAb（琼脂糖微球结合）可以用于Myc标签重组蛋白的免疫沉淀。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。Myc标为标签在细菌、酵母和哺乳动物细胞系统中广泛应用于检测重组蛋白的表达（1）。
原厂资料：
Specificity / Sensitivity
Myc-Tag (9B11) Mouse mAb detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy-termini of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Description
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Myc-Tag (9B111) Mouse mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of Myc-tagged recombinant proteins.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
注意事项：
Storage:Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
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