Phospho-c-Kit (Tyr719) Antibody

• 产品编号：CST-3391L      品牌：CST       原厂货号：3391L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

 磷酸化c-Kit(Tyr719)的抗体能检测内源性酪氨酸719位点被磷酸化c-Kit蛋白的表达水平。本抗体能与酪氨酸磷酸化的PDGF受体发生交叉反应。多克隆抗体通过用多肽免疫动物得到，该磷酸化多肽是根据小鼠的c-Kit蛋白Tyr719附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。c-Kit是受体酪氨酸激酶亚族的成员之一，这个家族包扩PDGF、 CSF-1和 FLT3/flk-2受体 (1,2)。它在造血干细胞、肥大细胞、黑色素细胞和生殖细胞等多种细胞的活化和生长过程中起重要作用(3)。通过和配体干细胞因子 (SCF)相结合，c-Kit发生二聚化/寡聚化并自磷酸化。激活的c-Kit能够招募下游含SH2信号传到成分，包括PLCγ、PI3K激酶的p85亚基、 SHP2和 CrkL，并使其酪氨酸磷酸化 (4)。导致c-Kit激酶功能减弱的分子损伤往往和各种发育异常相关(5)，而促使c-Kit组成型活化的突变则能导致肥大细胞增多症和胃肠道间质肿瘤(6)。Tyr719位于激酶催化插入区域。Tyr719位点磷酸化的c-Kit 的能够在体内或体外与PI3K激酶的p85亚基结合(7)。

1. Martin, F.H. et al. (1990) Cell 63, 203-11.
2. Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
3. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
4. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
5. Nocka, K. et al. (1990) EMBO J 9, 1805-13.
6. Hirota, S. et al. (1998) Science 279, 577-80.
7. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.

原厂资料：

Specificity / Sensitivity

Phospho-c-Kit (Tyr719) Antibody detects endogenous levels of c-Kit only when phosphorylated at tyrosine 719. The antibody cross-reacts with tyrosine-phosphorylated PDGF receptor.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr719 of mouse c-Kit. Antibodies are purified by protein A and peptide affinity chromatography.

Background

c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

1. Martin, F.H. et al. (1990) Cell 63, 203-11.
2. Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
3. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
4. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
5. Nocka, K. et al. (1990) EMBO J 9, 1805-13.
6. Hirota, S. et al. (1998) Science 279, 577-80.
7. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.