LPP (8B3A11) Mouse mAb

• 产品编号：CST-3389S      品牌：CST       原厂货号：3389S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LPP (8B3A11)小鼠单抗检测内源性总的LPP 蛋白水平。单抗由带HIS标签的重组片段的人LPP蛋白免疫动物产生。LIM包含型脂肪瘤特异的配体（LPP）属于zyxin家族，其成员包括LIMD1, ajuba, trip6 和 zyxin。在羧基末端的三个LIM结构域成为了此家族蛋白的特点。Zyxin家族成员与肌动蛋白细胞骨架相关，既是细胞间连接粘附复合物组分，又是整合素调节的粘附复合物组分（1）。它们在细胞核内外穿梭，在核内它们可能起着转录激活功能（1）。LPP 在细胞与细胞接触处绑定配体包括肌动蛋白调节因子α-actinin（2）和人类肿瘤抑制因子scrib，scrib调节细胞迁移和极性（3）。

1. Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
2. Hansen, M.D. and Beckerle, M.C. (2008) Biochem Biophys Res Commun 371, 144-8.
3. Petit, M.M. et al. (2005) BMC Cell Biol 6, 1.

原厂资料：

Specificity / Sensitivity

LPP (8B3A11) Mouse mAb detects endogenous levels of total LPP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a HIS-tagged recombinant fragment of human LPP.

Background

LIM-containing lipoma-preferred partner (LPP) belongs to the zyxin family, members of which include LIMD1, ajuba, trip6 and zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex (1). They shuttle in and out of the nucleus where they may function in transcriptional activation (1). 
 LPP binding partners at cell-cell contacts include the actin regulator α-actinin (2) and the human tumor suppressor scrib which regulates cell migration and polarity (3).

1. Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
2. Hansen, M.D. and Beckerle, M.C. (2008) Biochem Biophys Res Commun 371, 144-8.
3. Petit, M.M. et al. (2005) BMC Cell Biol 6, 1.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.