CDT1 Antibody

• 产品编号：CST-3386S      品牌：CST       原厂货号：3386S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDT1 Antibody能够检测内源性CDT1总蛋白。该多克隆抗体是由合成的人源的针对CDT1蛋白氨基末端序列的肽段免疫动物生产的。哺乳动物细胞的DNA复制启动是一个高度协调的过程，从而保证了每次细胞分裂中基因组只复制一次。复制的起源分散在整个基因组中，其活性主要通过前复制因子和复制因子的连续结合来调控。起始识别复合体(ORC)被认为可以结合整个细胞周期的染色质(1,2)。前复制复合体(Pre-RC)在有丝分裂末期/G1早期随着CDT1和cdc6的结合而形成，允许异源六聚化的MCM2-7复合体的结合。MCM复合体被认为是一种复制解旋酶，而pre-RC的形成参与染色质许可。随后的DNA复制起始需要S期促进激酶cdk2 和cdc7的激活。Cdc7(只有在复合体中伴随其调节亚基dbf4时才是活跃的)能够磷酸化结合染色质的MCM蛋白并允许复制因子cdc45和DNA聚合酶的结合(3,4)。CDT1结合geminin阻止了pre-RC的形成，而且geminin的表达和降解参与调节CDT1的活性(回顾5)。CDT1和MCM蛋白的相互作用在pre-RC的形成和认可过程中发挥重要作用(6,7)。Cdc6 和CDT1都被泛素蛋白酶体途径降解，以响应与再复制相关的DNA损伤(8)。

1. Okuno, Y. et al. (2001) EMBO J 20, 4263-77.
2. McNairn, A.J. et al. (2005) Exp Cell Res 308, 345-56.
3. Bell, S.P. and Dutta, A. (2002) Annu Rev Biochem 71, 333-74.
4. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
5. Tada, S. (2007) Front Biosci 12, 1629-41.
6. You, Z. and Masai, H. (2008) J Biol Chem 283, 24469-77.
7. Teer, J.K. and Dutta, A. (2008) J Biol Chem 283, 6817-25.
8. Hall, J.R. et al. (2008) J Biol Chem 283, 25356-63.

原厂资料：

Specificity / Sensitivity

CDT1 Antibody detects endogenous levels of total CDT1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminal sequence of human CDT1

Background

The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome, and their activities are regulated via the sequential binding of prereplication and replication factors. The origin recognition complex (ORC) is thought to be bound to chromatin throughout the cell cycle (1,2). The prereplication complex (Pre-RC) forms in late mitosis/early G1 phase beginning with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. The MCM complex is thought to be the replicative helicase, and formation of the pre-RC is referred to as chromatin licensing. Subsequent initiation of DNA replication requires the activation of the S-phase promoting kinases CDK2 and cdc7. Cdc7, which is active only in complex with its regulatory subunit dbf4, phosphorylates MCM proteins bound to chromatin and allows binding of the replication factor cdc45 and DNA polymerase (3,4). 
 Binding of CDT1 to geminin prevents pre-RC formation, and expression and degradation of geminin serve to regulate CDT1 activity (reviewed in 5). The interaction of CDT1 with MCM proteins is important in pre-RC formation and licensing (6,7). Both cdc6 and CDT1 are degraded by the ubiquitin proteasome pathway in response to DNA damage associated with rereplication (8).

1. Okuno, Y. et al. (2001) EMBO J 20, 4263-77.
2. McNairn, A.J. et al. (2005) Exp Cell Res 308, 345-56.
3. Bell, S.P. and Dutta, A. (2002) Annu Rev Biochem 71, 333-74.
4. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
5. Tada, S. (2007) Front Biosci 12, 1629-41.
6. You, Z. and Masai, H. (2008) J Biol Chem 283, 24469-77.
7. Teer, J.K. and Dutta, A. (2008) J Biol Chem 283, 6817-25.
8. Hall, J.R. et al. (2008) J Biol Chem 283, 25356-63.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody