Cathepsin B (H190) Antibody (Mouse Preferred)

• 产品编号：CST-3383S      品牌：CST       原厂货号：3383S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cathepsin B (H190) Antibody 兔多抗 (Mouse Preferred) 识别内源性的总cathepsin B蛋白。Cathepsin B (G60)Antibody 兔多抗#3373在Western Blot 中对人类Cathepsin B可以产生很强信号，Cathepsin B (H190) Antibody 兔多抗则更适合用于小鼠蛋白。该多克隆抗体由合成的人类cathepsin B蛋白His190位点附近肽段免疫动物而制成，部分区域与小鼠和大鼠蛋白保守同源。该抗体使用蛋白A和多肽亲和层析纯化而得。Cathepsin B（CSTB），木瓜蛋白酶家族的一部分，是一种广泛表达的溶酶体半胱氨酸的肽链内切酶（1,2）。Cathepsin B和其他家庭成员一样，由较大前体形式产生，其糖化形式在SDS-PAGE电泳上约为40 kDa的条带，由一个62个氨基酸的肽的裂解激活。研究发现在巨噬细胞和破骨细胞，以及不同类型的癌细胞，包括肺癌、结肠癌、前列腺癌、乳腺癌和胃癌中，cathepsin B高表达。此外，cathepsin B的表达与多发性硬化症（3）、类风湿关节炎（4）、胰腺炎（5）有关。虽然通常定位于溶酶体，癌变可能会导致其分泌（6）。其在肿瘤进展中的作用被认为是涉及促进基底膜降解、入侵和转移（7,8）。其表达与各种形式的癌症的预后较差相关（9-13）。

1. Chan, S.J. et al. (1986) Proc Natl Acad Sci USA 83, 7721-5.
2. Fong, D. et al. (1986) Proc Natl Acad Sci USA 83, 2909-13.
3. Bever, C.T. et al. (1994) Neurology 44, 745-8.
4. Hashimoto, Y. et al. (2001) Biochem Biophys Res Commun 283, 334-9.
5. Halangk, W. et al. (2000) J Clin Invest 106, 773-81.
6. Berquin, I.M. and Sloane, B.F. (1996) Adv Exp Med Biol 389, 281-94.
7. Yan, S. et al. (1998) Biol Chem 379, 113-23.
8. Vasiljeva, O. et al. (2006) Cancer Res 66, 5242-50.
9. Campo, E. et al. (1994) Am J Pathol 145, 301-9.
10. Foekens, J.A. et al. (1998) J Clin Oncol 16, 1013-21.
11. Werle, B. et al. (1999) Br J Cancer 81, 510-9.
12. Lah, T.T. et al. (2000) Clin Cancer Res 6, 578-84.
13. Werle, B. et al. (2000) Cancer 89, 2282-91.

原厂资料：

Specificity / Sensitivity

Cathepsin B (H190) Antibody (Mouse Preferred) detects endogenous levels of total cathepsin B protein. Cathepsin B (G60) Antibody #3373 produces a stronger signal for human cathepsin B on western blot, while Cathepsin B (H190) Antibody is preferred for the mouse protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His190 of human cathepsin B in a region that is conserved in mouse and rat. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cathepsin B (CSTB), part of the papain family of proteases, is a widely expressed lysosomal cysteine endopeptidase (1,2). Like other family members, cathepsin B is produced from a larger precursor form, pro-cathepsin B, which in its glycosylated form runs at approximately 40 kDa on SDS-PAGE, and is then activated by cleavage of a 62-amino acid pro-peptide. High levels of cathepsin B are found in macrophages and osteoclasts, as well as various types of cancer cells, including lung, colon, prostate, breast, and stomach. In addition, expression of cathepsin B has been associated with multiple sclerosis (3), rheumatoid arthritis (4), and pancreatitis (5). While generally localized to lysosomes, in cancer alterations can lead to its secretion (6). Its role in tumor progression is thought to involve promotion of basement membrane degradation, invasion and metastasis (7,8). Expression can correlate with poor prognosis for a variety of forms of cancer (9-13).

1. Chan, S.J. et al. (1986) Proc Natl Acad Sci USA 83, 7721-5.
2. Fong, D. et al. (1986) Proc Natl Acad Sci USA 83, 2909-13.
3. Bever, C.T. et al. (1994) Neurology 44, 745-8.
4. Hashimoto, Y. et al. (2001) Biochem Biophys Res Commun 283, 334-9.
5. Halangk, W. et al. (2000) J Clin Invest 106, 773-81.
6. Berquin, I.M. and Sloane, B.F. (1996) Adv Exp Med Biol 389, 281-94.
7. Yan, S. et al. (1998) Biol Chem 379, 113-23.
8. Vasiljeva, O. et al. (2006) Cancer Res 66, 5242-50.
9. Campo, E. et al. (1994) Am J Pathol 145, 301-9.
10. Foekens, J.A. et al. (1998) J Clin Oncol 16, 1013-21.
11. Werle, B. et al. (1999) Br J Cancer 81, 510-9.
12. Lah, T.T. et al. (2000) Clin Cancer Res 6, 578-84.
13. Werle, B. et al. (2000) Cancer 89, 2282-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.