Phospho-Erk5 (Thr218/Tyr220) Antibody

• 产品编号：CST-3371S      品牌：CST       原厂货号：3371S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Erk5 (Thr218/Tyr220) Antibody兔多抗能检测免疫沉淀和转染水平的苏氨酸（218位点）、酪氨酸（220位点）磷酸化的Erk5蛋白。该抗体能与磷酸化的Erk1 、Erk2蛋白发生交叉反应，但不与磷酸化的p38 MAPK 、SAPK/JNK发生交叉反应。该单克隆抗体是通过用与人源Erk5蛋白苏氨酸（218位点）/酪氨酸（220位点）周围相一致的磷酸化合成肽段免疫动物后获得的。该抗体经蛋白A和肽亲和层析纯化。ERK5（有丝分裂原激活蛋白激酶7，大有丝分裂原激活蛋白激酶1）是MAPK超家族的一员，调控了多种细胞过程包括细胞增殖，分化和存活(1,5-7)。和其他MAPK家族成员一样，ERK5含有一个经典的活化环TEY基序（Thr218/Tyr220)，能以生长因子依赖、Ras非依赖的机制被MAP2K5 (MEK5)磷酸化(2-4)。例如，EGF刺激促进了ERK5的磷酸化，这会诱导它转位到细胞核，随后磷酸化MEF2C和其他转录靶点（2,3）。ERK5也可以被造血干细胞中的粒细胞集落刺激因子(G-CSF)所激活，在造血干细胞中促进细胞的存活和扩增（4）。在神经细胞中，ERK5是NGF-诱导的神经突增生、神经稳态和神经存活所必需的（11,12）。ERK5被认为在维持血管稳定性发挥了作用，它能保持内皮细胞迁移和其屏障作用（8-10)。尽管表达广泛，但研究表明缺乏erk5的小鼠会表现出多种心脏缺陷，提示ERK5在血管发育和稳态过程中发挥了至关重要的作用（1,5）。

1. Zhou, G. et al. (1995) J Biol Chem 270, 12665-9.
2. Hayashi, M. and Lee, J.D. (2004) J Mol Med 82, 800-8.
3. Wang, X. and Tournier, C. (2006) Cell Signal 18, 753-60.
4. Nishimoto, S. and Nishida, E. (2006) EMBO Rep 7, 782-6.
5. Kato, Y. et al. (1998) Nature 395, 713-6.
6. Kato, Y. et al. (1997) EMBO J 16, 7054-66.
7. Dong, F. et al. (2001) J Biol Chem 276, 10811-6.
8. Obara, Y. et al. (2009) J Biol Chem 284, 23564-73.
9. Finegan, K.G. et al. (2009) Cell Death Differ 16, 674-83.
10. Spiering, D. et al. (2009) J Biol Chem 284, 24972-80.
11. Sawhney, R.S. et al. (2009) J Cell Physiol 219, 152-61.
12. Zhao, Z. et al. (2009) Mol Cell Biochem 322, 171-8.

原厂资料：

Specificity / Sensitivity

Phospho-Erk5 (Thr218/Tyr220) Antibody detects immunoprecipitated or transfected levels of Erk5 phosphorylated at threonine 218 and tyrosine 220. This antibody cross-reacts with phosphorylated Erk1 and Erk2. It does not cross-react with phosphorylated p38 MAPK or SAPK/JNK.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr218/Tyr220 of human Erk5. Antibodies are purified by protein A and peptide affinity chromatography.

Background

ERK5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation and survival (1,5-7). Like other MAPK family members, ERK5 contains a canonical activation loop TEY motif (Thr218/Tyr220) which is specifically phosphorylated by MAP2K5 (MEK5) in a growth factor-dependent, Ras-independent mechanism (2-4). For example, EGF stimulation promotes ERK5 phosphorylation which induces its traslocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (2,3). ERK5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoetic progenitor cells where is promotes survival and proliferation (4). In neuronal cells, ERK5 is required for NGF-induced neurite outgrowth neuronal homeostatis and survival (11,12). ERK5 is thought to play a role in blood vessel integrity via maintainence of endothelial cell migration and barrier function (8-10). Although broadly expressed, mice lacking erk5 display numerous cardiac defects, suggesting ERK5 plays a critical role in vascular development and homeostasis (1,5).

1. Zhou, G. et al. (1995) J Biol Chem 270, 12665-9.
2. Hayashi, M. and Lee, J.D. (2004) J Mol Med 82, 800-8.
3. Wang, X. and Tournier, C. (2006) Cell Signal 18, 753-60.
4. Nishimoto, S. and Nishida, E. (2006) EMBO Rep 7, 782-6.
5. Kato, Y. et al. (1998) Nature 395, 713-6.
6. Kato, Y. et al. (1997) EMBO J 16, 7054-66.
7. Dong, F. et al. (2001) J Biol Chem 276, 10811-6.
8. Obara, Y. et al. (2009) J Biol Chem 284, 23564-73.
9. Finegan, K.G. et al. (2009) Cell Death Differ 16, 674-83.
10. Spiering, D. et al. (2009) J Biol Chem 284, 24972-80.
11. Sawhney, R.S. et al. (2009) J Cell Physiol 219, 152-61.
12. Zhao, Z. et al. (2009) Mol Cell Biochem 322, 171-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.