Neuropilin-2 (D39A5) XP? Rabbit mAb

• 产品编号：CST-3366P      品牌：Cell Signaling Technology       原厂货号：3366P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Neuropilin-2 (D39A5) XP® Rabbit mAb 兔单抗识别内源性的neuropilin-2总蛋白。该单克隆抗体由重组的小鼠Neuropilin-2蛋白氨基末端蛋白免疫动物而制成。Class 3 secreted semaphorin (Sema3A)是多种轴突上的神经生长导向因子。因此，它可以诱导微丝发生戏剧性的再分布和解聚，导致生长锥崩塌。Plexins是单次跨膜信号转导蛋白，包括Plexin A1，A2，A3和A4。Plexins可以与neuropilin-1、neuropilin-2和细胞黏附蛋白L1形成功能性的semaphorin受体（1,2）。GTP酶Rnd1结合Plexin A1的细胞质结构域，触发细胞骨架崩溃。相反，GTP酶RndD阻止Rnd1-介导的Plexin A1的活性和Sema3A所致交感神经轴突的排斥（3）。Neuropilin-2是一个单次跨膜的糖蛋白，也可以结合VEGF165和VEGF145，但不能结合VEGF121。所以，除了神经导向，neuropilin-2在血管生成和心血管功能中扮演重要作用（4）。尽管neuropilin-2与肿瘤有关（如胰腺癌、膀胱癌、肺癌、神经母细胞瘤），其在肿瘤中的致病机理目前尚未阐明（4,5）。

1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
4. Favier, B. et al. (2006) Blood 108, 1243-50.
5. Chen, C. et al. (2005) World J Surg 29, 271-5.

原厂资料：

Specificity / Sensitivity

Neuropilin-2 (D39A5) XP® Rabbit mAb detects endogenous levels of total neuropilin-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse Neuropilin-2 protein.

Background

Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3 and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).Neuropilin-2, a single pass, transmembrane glycoprotein, can also bind to VEGF165 and VEGF145, but not VEGF121. Thus, in addition to neuronal guidance, neuropilin-2 plays a role in angiogenesis and cardiovascular functions (4). Although neuropilin-2 has been linked to tumor biology (e.g. pancreatic cancer, bladder cancer, lung cancer, and neuroblastoma), its role in tumor pathogenesis is still under investigation (4,5).

1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
4. Favier, B. et al. (2006) Blood 108, 1243-50.
5. Chen, C. et al. (2005) World J Surg 29, 271-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.