Drosha (D28B1) Rabbit mAb

• 产品编号：CST-3364S      品牌：CST       原厂货号：3364S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Drosha (D28B1) Rabbit mAb兔单抗检测内源性Drosha总蛋白水平。通过人工合成人源Drosha蛋白Gly953位点周围序列相应的多肽片段，使用该片段去免疫动物从而制备单克隆抗体。研究显示Drosha作为一个核内的III类RNase，它能能够催化microRNA (miRNA)加工的起始阶段(1)。该酶具有将长的初始转录本pri-miRNAs加工成含有茎环结构的pre-miRNA。干扰Drosha将导致pri-miRNAs的增加和pre-miRNAs的减少(1)。Drosha存在于称为微加工器复合物的多蛋白复合物中，该复合物中还有其它组分如DGCR8蛋白(2)。Drosha与DGCR8蛋白一起，对于miRNA生物合成是必须的(3)。

1. Lee, Y. et al. (2003) Nature 425, 415-9.
2. Denli, A.M. et al. (2004) Nature 432, 231-5.
3. Gregory, R.I. et al. (2004) Nature 432, 235-40.

原厂资料：

Specificity / Sensitivity

Drosha (D28B1) Rabbit mAb detects endogenous levels of total Drosha protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly953 of human Drosha.

Background

Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components such as DGCR8 (2). Drosha, along with DGCR8, is necessary for miRNA biogenesis (3).

1. Lee, Y. et al. (2003) Nature 425, 415-9.
2. Denli, A.M. et al. (2004) Nature 432, 231-5.
3. Gregory, R.I. et al. (2004) Nature 432, 235-40.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.