Phospho-CaMKII (Tyr231) Antibody

• 产品编号：CST-3356S      品牌：CST       原厂货号：3356S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-CaMKII (Tyr231) Antibody 兔多抗识别内源性的Tyr231磷酸化的CaMKII蛋白。该多克隆抗体由合成的人类CaMKII蛋白Tyr231位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。CaMKII是一种钙/钙调素活化蛋白激酶家族的重要成员，在神经突触的刺激和T细胞受体信号通路中起作用（1,2）。CaMKII具有催化和调节功能域。与Ca2+/钙调蛋白调节功能域的结合，可以产生自身抑制效果，并激活激酶（3）。活化的CaMKII进一步在Thr286自身磷酸化，呈现出其具有的激酶活性（3）。CaMKII的苏氨酸磷酸化状态，可通过PP1/PKA调节。PP1（蛋白磷酸酶1）可以对磷酸化的CaMKII在Thr286进行去磷酸化。PKA（蛋白激酶A）通过对PP1的抑制作用防止这种去磷酸化（4）。Phospho-CaMKII (Tyr231)Antibody 兔多抗用来直接识别前述未发表的CaMKII 酪氨酸磷酸化位点Tyr231。它们由CST公司使用PhosphoScan®和CST磷酸化位点发现的质谱平台鉴定。CamKII在Tyr231上的磷酸化在缺血大鼠脑组织分离提取物中可见。

1. Hughes, K. et al. (2001) J Biol Chem 276, 36008-13.
2. Barria, A. et al. (1997) Science 276, 2042-5.
3. Barkai, U. et al. (2000) Mol Endocrinol 14, 554-63.
4. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

原厂资料：

Specificity / Sensitivity

Phospho-CaMKII (Tyr231) Antibody detects endogenous levels of CaMKII only when phosphorylated at Tyr231.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr231 of human CaMKII. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca2+/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4).Phospho-CaMKII (Tyr231) Antibody is directed against a previously unpublished CaMKII tyrosine phosphorylation site at Tyr231 that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of CamKII at Tyr231 was observed in extracts isolated from ischemic rat brain.

1. Hughes, K. et al. (2001) J Biol Chem 276, 36008-13.
2. Barria, A. et al. (1997) Science 276, 2042-5.
3. Barkai, U. et al. (2000) Mol Endocrinol 14, 554-63.
4. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody