CACYBP Antibody

• 产品编号：CST-3354S      品牌：CST       原厂货号：3354S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CACYBP抗体识别内源性的总CACYBP蛋白。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源CACYBP蛋白的 Trp162氨基酸残基序列一致，抗体由蛋白A和肽段亲和层析技术纯化。钙周期蛋白结合蛋白或Siah-1相互作用蛋白(CACYBP/SIP)是泛素E3连接酶复合物成员之一，其他成员Siah1, Skp1和 Ebi（1）。CACYBP调控β-catenin更新和对胸腺细胞发育起重要作用（2）。CACYBP也和tubulin结合可能涉及细胞骨架调控（3，4）。它在神经元里高表达，它的细胞定位可能被Ca2+调控（5,6）。视黄酸处理的成神经瘤细胞系SH-SY5Y，会诱使CACYBP的转位入核，可能与CACYBP在丝氨酸残基的磷酸化相关（7）。近来研究也暗示CACYBP可能具有磷酸酶活性（8）并可能结合并去磷酸化Erk1/2（8,9）。

1. Matsuzawa, S.I. and Reed, J.C. (2001) Mol Cell 7, 915-26.
2. Fukushima, T. et al. (2006) Immunity 24, 29-39.
3. Schneider, G. et al. (2007) Biochim Biophys Acta 1773, 1628-36.
4. Filipek, A. et al. (2008) J Neural Transm 115, 1257-64.
5. Jastrzebska, B. et al. (2000) J Histochem Cytochem 48, 1195-202.
6. Filipek, A. et al. (2002) J Biol Chem 277, 21103-9.
7. Wu, J. et al. (2003) J Biochem Mol Biol 36, 354-8.
8. Kilanczyk, E. et al. (2011) Biochem Biophys Res Commun 404, 179-83.
9. Kilanczyk, E. et al. (2009) Biochem Biophys Res Commun 380, 54-9.

原厂资料：

Specificity / Sensitivity

CACYBP Antibody detects endogenous levels of total CACYBP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp162 of human CACYBP. Antibodies are purified by peptide affinity chromatography.

Background

Calcyclin-binding protein or Siah-1-interacting protein (CACYBP/SIP) is a component of the ubiquitin E3 ligase complex that also contains Siah1, Skp1, and Ebi (1). CACYBP regulates β-catenin turnover and plays an important role in thymocyte development (2). CACYBP also binds to tubulin and may be involved in cytoskeletal regulation (3,4). It is highly expressed in neurons, and its cellular localization may be regulated by Ca2+ (5,6). Retinoic acid treatment of the neuroblastoma cell line SH-SY5Y induces translocation of CACYBP to the nucleus and seems to be correlated with phosphorylation of CACYBP on serine residues (7). Recent studies also suggest that CACYBP may possess phosphatase activity (8), and that it can bind and dephosphorylate Erk1/2 (8,9).

1. Matsuzawa, S.I. and Reed, J.C. (2001) Mol Cell 7, 915-26.
2. Fukushima, T. et al. (2006) Immunity 24, 29-39.
3. Schneider, G. et al. (2007) Biochim Biophys Acta 1773, 1628-36.
4. Filipek, A. et al. (2008) J Neural Transm 115, 1257-64.
5. Jastrzebska, B. et al. (2000) J Histochem Cytochem 48, 1195-202.
6. Filipek, A. et al. (2002) J Biol Chem 277, 21103-9.
7. Wu, J. et al. (2003) J Biochem Mol Biol 36, 354-8.
8. Kilanczyk, E. et al. (2011) Biochem Biophys Res Commun 404, 179-83.
9. Kilanczyk, E. et al. (2009) Biochem Biophys Res Commun 380, 54-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.