Ras (27H5) Rabbit mAb

• 产品编号：CST-3339S      品牌：CST       原厂货号：3339S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Ras (27H5) Rabbit mAb兔单抗能检测内源性总K-Ras, H-Ras 和 N-Ras蛋白水平。该单克隆抗体是采用合成的与人源K-Ras蛋白胺端周围残基相一致的磷酸化肽段免疫动物后获得的。21kDa的鸟苷酸结合蛋白(K-Ras, H-Ras, and N-Ras)在活化状态(GTP-bound)和失活状态(GDP-bound)不断转换（1）。受体酪氨酸激酶和G-蛋白偶联受体能够激活Ras，随后刺激Raf-MEK-MAPK信号通路(2-4)。GAP蛋白通常能够促进Ras的失活。但是，有研究表明，在30%的人类肿瘤中，Ras的点突变会阻止GAP介导的对该条通路的抑制作用（5）。肿瘤中最常见的致癌性Ras突变是Gly12突变为Asp（G12D），这会抑制Ras的失活，可能是通过增加蛋白的刚性而实现的(5,6）。

1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-54.
2. Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83.
3. Buday, L. and Downward, J. (1993) Cell 73, 611-20.
4. Huang, D.C. et al. (1993) Mol Cell Biol 13, 2420-31.
5. Bos, J.L. (1989) Cancer Res 49, 4682-9.
6. Ma, J. and Karplus, M. (1997) J Mol Biol 274, 114-31.

原厂资料：

Specificity / Sensitivity

Ras (27H5) Rabbit mAb detects endogenous levels of total K-Ras, H-Ras and N-Ras proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human K-Ras.

Background

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-54.
2. Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83.
3. Buday, L. and Downward, J. (1993) Cell 73, 611-20.
4. Huang, D.C. et al. (1993) Mol Cell Biol 13, 2420-31.
5. Bos, J.L. (1989) Cancer Res 49, 4682-9.
6. Ma, J. and Karplus, M. (1997) J Mol Biol 274, 114-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.