Phospho-Jak1 (Tyr1034/1035) Antibody

• 产品编号：CST-3331S      品牌：CST       原厂货号：3331S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Jak1 (Tyr1022/1023) Antibody只能检测内源的在tyr1022/1023位点磷酸化的Jak1蛋白。此抗体可能与phospho-Jak2交叉反应。此多克隆抗体是通过合成人源对应的Jak1 Tyr1022/1023位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。Syk 家族酪氨酸激酶Zap-70 在T 细胞和 NK 细胞中表达并在响应T细胞受体(TCR)而介导T细胞激活中发挥了重要的作用(1)。在TCR 招募下, Zap-70 通过自磷酸化和Src家族酪氨酸激酶Lck转磷酸化作用下快速的磷酸化几个酪氨酸残基(2-6)。酪氨酸的磷酸化与Zap-70激酶活性的增加和下游信号传导有关系。Zap-70 的表达与疾病的进程和慢性淋巴白血病患者的生存率有关(7,8)。Jak1酪氨酸残基1022/1023存在于可能的活性环中的与Tyk2中的酪氨酸残基1054/1055属于同源残基，这两个残基对Tyk2激酶活性的调节有重要的作用(6)。(此产品的销售具有来自Chemicon, Inc., U.S. Patent No. 5,658,791的许可。)

1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
2. Darnell, J.E. (1997) Science 277, 1630-5.
3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
6. Gauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.

原厂资料：

Specificity / Sensitivity

Phospho-Jak1 (Tyr1022/1023) Antibody detects endogenous levels of Jak1 only when phosphorylated at tyrosines 1022/1023. This antibody may cross-react with phospho-Jak2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1022/1023 of human Jak1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

The tyrosine residues 1022/1023 of Jak1 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6). 
 
 (This product is sold under license from Chemicon, Inc., U.S. Patent No. 5,658,791.)

1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
2. Darnell, J.E. (1997) Science 277, 1630-5.
3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
6. Gauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.