PTPA/PPP2R4 Antibody

• 产品编号：CST-3330S      品牌：CST       原厂货号：3330S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

.PTPA/PPP2R4 Antibody 能够检测内源性PTPA总蛋白。该多克隆抗体是由合成的人源的针对PTPA蛋白的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。蛋白磷酸酶2A (PP2A)是一种重要的蛋白丝氨酸/苏氨酸磷酸酶，在所有的真核细胞中结构保守。PP2A在各种细胞信号转导通路中都是关键的酶，它调节基本的细胞活动如DNA复制、转录、翻译、代谢、细胞周期进程、细胞分化、细胞凋亡和发育(1-3)。活性蛋白磷酸酶2A是由结构亚基A和催化亚基C组成，其活性依赖于和B亚基的相互作用。一个重要的PP2A调节亚基是PP2A磷酸酶活化剂(PTPA)，亦被称为PP2A活化剂调节亚基4(PPP2R4) (4)。该PTPA调节蛋白结合ATP且具有异构酶(PPIase)活性，表明PP2A调节涉及磷酸酶的构象改变。ATP和Mg2+的增加导致相关的PP2A激活增加和PTPA异构酶活性增加(5)。尽管具体的机制尚在研究中，有证据表明PTPA结合到PP2A A-C二聚体能够导致PP2A的构象变化，改变磷酸酶底物特异性从磷酸丝氨酸到磷酸酪氨酸底物(6)。

1. Janssens, V. and Goris, J. (2001) Biochem J 353, 417-39.
2. Zolnierowicz, S. (2000) Biochem Pharmacol 60, 1225-35.
3. Millward, T.A. et al. (1999) Trends Biochem Sci 24, 186-91.
4. Fellner, T. et al. (2003) Genes Dev 17, 2138-50.
5. Jordens, J. et al. (2006) J Biol Chem 281, 6349-57.
6. Chao, Y. et al. (2006) Mol Cell 23, 535-46.

原厂资料：

Specificity / Sensitivity

PTPA/PPP2R4 Antibody detects endogenous levels of total PTPA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PTPA. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). Active protein phosphatase 2A is composed of both structural (A) and catalytic (C) proteins, and its activity relies on interaction with regulatory (B) subunits. An important PP2A regulatory subunit is PP2A phosphatase activator (PTPA), also known as the PP2A activator regulatory subunit 4 (PPP2R4) (4). This PTPA regulatory protein binds ATP and has isomerase (PPIase) activity, suggesting that PP2A regulation involves a change in phosphatase conformation. The addition of ATP (and Mg2+) results in a correlated increase in both PP2A activation and PTPA isomerase activity (5). While the exact mechanism is still under consideration, evidence suggests that binding of PTPA to the PP2A A-C dimer produces a conformational change in PP2A that shifts phosphatase substrate specificity from phosphoserine to phosphotyrosine substrates (6).

1. Janssens, V. and Goris, J. (2001) Biochem J 353, 417-39.
2. Zolnierowicz, S. (2000) Biochem Pharmacol 60, 1225-35.
3. Millward, T.A. et al. (1999) Trends Biochem Sci 24, 186-91.
4. Fellner, T. et al. (2003) Genes Dev 17, 2138-50.
5. Jordens, J. et al. (2006) J Biol Chem 281, 6349-57.
6. Chao, Y. et al. (2006) Mol Cell 23, 535-46.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.