Phospho-Dab1 (Tyr220) Antibody

• 产品编号：CST-3327S      品牌：CST       原厂货号：3327S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Dab1 (Tyr220) Antibody 兔多抗识别转染的Tyr220磷酸化的Dab1蛋白。该多克隆抗体由合成的人类Dab1蛋白Tyr220位点附近肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。Reelin信号转导通路在神经发育中起着关键作用。Reelin是一种分泌糖蛋白，可以和神经元表面的极低密度脂蛋白受体、ApoER2或α3β1整联蛋白结合（1,2）。这些受体激活诱导胞内接头蛋白Disabled 1 (Dab1)的酪氨酸磷酸化。普遍认为，Src家族酪氨酸激酶对Dab1酪氨酸磷酸化是Reelin信号下游事件中最关键的。其氨基酸末端的磷酸酪氨酸连接（PTB）域使Dab1识别和连接到受体胞质段尾部的保守序列模体上。另外，PTB包含了一个能结合磷酸肌醇的Pleckstrin同源结合子域。Dab1 PTB域内磷酸肌醇结合区域是膜定位和独立于VLDLR和ApoER2时基础酪氨酸磷酸化所必需的。研究显示Src, CrkII, CrkL和Dock1与络氨酸磷酸化的Dab有联系。CrkII-Dab1相互作用需要Dab1在220或232处进行络氨酸磷酸化。

1. Huang, Y. et al. (2005) Biochem. Biophys. Res. Commun. 331, 1460-1468.
2. Luque, J.M. (2004) Brain Res. Dev. Brain Res. 152, 269-271.
3. Morimura, T. et al. (2005) J. Biol. Chem. 280, 16901-16908.
4. Chen, K. et al. (2004) J. Cell. Sci. 117, 4527-4536.

原厂资料：

Specificity / Sensitivity

Phospho-Dab1 (Tyr220) Antibody detects transfected levels of Dab1 protein only when phosphorylated at tyrosine 220.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr220 of human Dab1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Reelin signaling pathway plays a critical role in neuronal development. Reelin is a secreted glycoprotein that binds to the lipoprotein receptors VLDLR and ApoER2 or alpha3beta1 integrin on the surface of neurons (1,2). Activation of these receptors induces tyrosine phosphorylation of Disabled 1 (Dab1), an intracellular adaptor. It is generally believed that tyrosine phosphorylation of Dab1 by Src family tyrosine kinases is the most critical downstream event in Reelin signaling. The phosphotyrosine-binding (PTB) domain within its amino terminus enables Dab1 to recognize and bind to a conserved sequence motif within the cytoplasmic tail of the receptors. In addition, the PTB contains a Pleckstrin Homology-like subdomain that binds to phosphoinositides. The phosphoinositide-binding region within the Dab1 PTB domain is required for membrane localization and basal tyrosine phosphorylation of Dab1 independent of VLDLR and ApoER2 (3). It has been demonstrated that Src, CrkII, CrkL and Dock1 associate with tyrosine-phosphorylated Dab. The CrkII-Dab1 interaction requires tyrosine phosphorylation of Dab1 at residues 220 or 232 (4).

1. Huang, Y. et al. (2005) Biochem. Biophys. Res. Commun. 331, 1460-1468.
2. Luque, J.M. (2004) Brain Res. Dev. Brain Res. 152, 269-271.
3. Morimura, T. et al. (2005) J. Biol. Chem. 280, 16901-16908.
4. Chen, K. et al. (2004) J. Cell. Sci. 117, 4527-4536.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.